铜绿丽金龟对寄主植物挥发物的触角电生理及行为反应

为深入了解铜绿丽金龟的取食行为,探索开发安全高效的植物源引诱剂,应用昆虫触角电位反应仪和Y型嗅觉仪测试其对不同寄主植物挥发物的趋向行为差异,并根据室内行为结果配制诱剂进行大田试验。结果表明:铜绿丽金龟雄虫对水杨酸甲酯和(1,1’-联环戊基)-2-酮的触角电位反应(electroantennography,EAG)值显著高于其它试剂;石竹烯和水杨酸甲酯能引起雌虫触角较强的电位反应;乙酸顺式-3-己烯酯和石竹烯分别对雄虫和雌虫有较高的嗅觉选择反应率,分别达0.95和0.94。综合EAG和嗅觉试验结果,选择对雌、雄虫均有较好引诱效果的反式-2-己烯醛、乙酸顺式-3-己烯酯、石竹烯、(1,1’-联环戊基)-2-酮和水杨酸甲酯进行田间试验,最终筛选到铜绿丽金龟雄虫的最适引诱剂为每诱芯360 mg乙酸顺式-3-己烯酯,雌虫的最适引诱剂为每诱芯360 mg石竹烯,日诱虫量分别可达33.00±1.53头和29.33±1.45头。

EAG and behavioral responses of copper-green chafer Anomala corpulenta Motschulsky (Coleoptera: Rutelidae)

In order to understand the feeding behavior of Anomala corpulenta (Coleoptera), explore safe and efficient plant-derived attractant, the behavioral responses of A. corpulenta to odors from leaves of different host plants were tested by using electroantennography (EAG), Y-shaped olfactometer and field trapping test. The results showed that the EAG value of A. corpulenta males to 2-carbomethoxyphenol and 1,1'-bi (cyclopentyl)-2-one were significantly higher than to the other chemicals tested; the EAG value of females to (-)-E-caryophyllene and 2-carbomethoxyphenol were significantly higher than to other reagents. According to the Y-shaped bioassays, (Z)-3-hexenyl acetate and (-)-E-caryophyllene were found to be more attractive to males and females of A. corpulenta, respectively. The results of field trapping test with (E)-2-hexenal, (Z)-3-hexenyl acetate, (-)-E-caryophyllene, 1,1'-bi(cyclopentyl)-2-one and 2-carbomethoxyphenol showed that cis-3-hexenyl acetate (360 mg/lure) attracted the highest number (33±1.53) of males, while (-)-E-caryophyllene (360 mg/lure) attracted the highest number (29.33±1.45) of females.