| 群体感应调控因子ComA对枯草芽胞杆菌NCD-2抑菌物质产生和生物膜形成的影响 |
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| 引用本文： | 王培培,郭庆港,李社增,鹿秀云,张晓云,马平. 群体感应调控因子ComA对枯草芽胞杆菌NCD-2抑菌物质产生和生物膜形成的影响[J]. 植物保护学报, 2016, 43(2): 193-200. DOI: 10.13802/j.cnki.zwbhxb.2016.02.003 |
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| 作者姓名： | 王培培 郭庆港 李社增 鹿秀云 张晓云 马平 |
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| 作者单位： | 1. 河北农业大学,保定071000;河北省农林科学院植物保护研究所,河北省农业有害生物综合防治工程技术研究中心,农业部华北北部作物有害生物综合治理重点实验室,保定071000;2. 河北省农林科学院植物保护研究所,河北省农业有害生物综合防治工程技术研究中心,农业部华北北部作物有害生物综合治理重点实验室,保定071000 |
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| 基金项目： | 国家自然科学基金(31272085),国家"863"计划(2011AA10A205),国家公益性行业(农业)科研专项(201303025) |
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| 摘 要： | 为明确群体感应系统在枯草芽胞杆菌NCD-2菌株抑菌活性和生物膜形成中的作用,通过同源重组技术对群体感应系统中的comA基因进行定位缺失突变,分别比较了NCD-2菌株和comA基因突变子在胞外酶合成、抑菌活性、脂肽抗生素(丰产素)产生以及生物膜形成上的差异,并进一步利用实时荧光定量RT-PCR技术比较了二者生物膜基质编码基因epsA和tasA的表达情况。结果表明,通过同源重组技术获得了comA基因突变子MA-4,同菌株NCD-2相比,其在胞外蛋白酶和纤维素酶的合成能力、对番茄灰霉菌Botrytis cinerea的抑菌活性、丰产素的合成量和生物膜的形成能力上均明显下降;生物膜基质编码基因tasA的表达量下降了70%,而epsA的表达量变化不明显。表明ComA是NCD-2菌株脂肽抗生素和生物膜形成中的重要调控因子。
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| 关 键 词： | 枯草芽胞杆菌 调控因子 脂肽抗生素 生物膜 |
| 收稿时间： | 2014-10-13 |
Functional analysis of quorum sensing regulator ComA on the production of antifungal active compounds and biofilm formation in Bacillus subtilis NCD-2 |
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| Wang Peipei,Guo Qinggang,Li Shezeng,Lu Xiuyun,Zhang Xiaoyun and Ma Ping. Functional analysis of quorum sensing regulator ComA on the production of antifungal active compounds and biofilm formation in Bacillus subtilis NCD-2[J]. Acta Phytophylacica Sinica, 2016, 43(2): 193-200. DOI: 10.13802/j.cnki.zwbhxb.2016.02.003 |
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| Authors: | Wang Peipei Guo Qinggang Li Shezeng Lu Xiuyun Zhang Xiaoyun Ma Ping |
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| Affiliation: | Agricultural University of Hebei, Baoding 071000, Hebei Province, China;Key Laboratory of IPM on Crops in Northern Region of North China, Ministry of Agriculture; Integrated Pest Management Center of Hebei Province; Institute of Plant Protection, Hebei Academy of Agricultural and Forestry Sciences, Baoding 071000, Hebei Province, China,Key Laboratory of IPM on Crops in Northern Region of North China, Ministry of Agriculture; Integrated Pest Management Center of Hebei Province; Institute of Plant Protection, Hebei Academy of Agricultural and Forestry Sciences, Baoding 071000, Hebei Province, China,Key Laboratory of IPM on Crops in Northern Region of North China, Ministry of Agriculture; Integrated Pest Management Center of Hebei Province; Institute of Plant Protection, Hebei Academy of Agricultural and Forestry Sciences, Baoding 071000, Hebei Province, China,Key Laboratory of IPM on Crops in Northern Region of North China, Ministry of Agriculture; Integrated Pest Management Center of Hebei Province; Institute of Plant Protection, Hebei Academy of Agricultural and Forestry Sciences, Baoding 071000, Hebei Province, China,Key Laboratory of IPM on Crops in Northern Region of North China, Ministry of Agriculture; Integrated Pest Management Center of Hebei Province; Institute of Plant Protection, Hebei Academy of Agricultural and Forestry Sciences, Baoding 071000, Hebei Province, China and Key Laboratory of IPM on Crops in Northern Region of North China, Ministry of Agriculture; Integrated Pest Management Center of Hebei Province; Institute of Plant Protection, Hebei Academy of Agricultural and Forestry Sciences, Baoding 071000, Hebei Province, China |
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| Abstract: | |
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| Keywords: | Bacillus subtilis regulatory factor lipopeptide antibiotic biofilm |
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