| 文心兰高效再生体系研究 |
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| 引用本文： | 赵丽,田维敏. 文心兰高效再生体系研究[J]. 安徽农业科学, 2008, 36(20) |
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| 作者姓名： | 赵丽 田维敏 |
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| 作者单位： | 中国热带农业科学院橡胶研究所,海南儋州,571737;中国热带农业科学院橡胶研究所,海南儋州,571737 |
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| 摘 要： | 以文心兰试管苗茎尖为外植体,对不同类型培养基诱导原球茎、增殖及分化成苗的效果进行了研究。结果表明,文心兰茎尖在MS+6-BA 6.00 mg/L+NAA 0.15 mg/L培养基上,1个月后就能诱导大量原球茎,培养基MS+6-BA 0.50 mg/L+NAA 0.15 mg/L+5%椰子水有利于芽的诱导,培养基1/2 MS+IBA 1.00 mg/L+10%椰子水有利于诱导生根。
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| 关 键 词： | 文心兰 原球茎 再生体系 |
Study on the High-efficient Regeneration System of Oncidium |
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| ZHAO Li et al. Study on the High-efficient Regeneration System of Oncidium[J]. Journal of Anhui Agricultural Sciences, 2008, 36(20) |
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| Authors: | ZHAO Li et al |
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| Abstract: | The different media were screened on the basis of their effects on the induction of protocorm-like body and bud,as well as regeneration and rooting of Oncidium seedlings.The results showed that protocorm-like body could be introduced from stem tip explants in the MS medium containing 6-BA(6.00 mg/L) NAA(0.15 mg/L) after one month and the medium MS 0.50 mg/L BA 0.15 mg/L NAA 5% coconut juice and 1/2 MS 1.00 mg/L IBA 10% coconut juice were suitable for bud regeneration and rooting,respectively. |
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| Keywords: | Oncidium Protocorm-like body Regeneration system |
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