| 生物表面活性剂排油圈检测方法的改进和应用 |
| |
| 引用本文： | 毕思宁,王彦杰,左豫虎.生物表面活性剂排油圈检测方法的改进和应用[J].黑龙江八一农垦大学学报,2009,21(6):58-60. |
| |
| 作者姓名： | 毕思宁 王彦杰 左豫虎 |
| |
| 作者单位： | 1. 黑龙江八一农垦大学农学院,大庆,163319
2. 黑龙江八一农垦大学生命科学技术学院 |
| |
| 基金项目： | 高等学校博士学科点专项科研基金资助项目，黑龙江省教育厅项目 |
| |
| 摘 要： | 本研究对生物表面活性剂排油圈检测方法进行改进,将原方法中所用液体石蜡加入苏丹红Ⅲ染色,改进后方法的测定结果与原方法结果相比差异不显著,并且试验效果对比明显,容易观察,可以实时拍照。方法操作简单、测定结果准确,很有实际应用价值。采用本方法测定了供试菌株BS1不同浓度发酵液的排油活性,结果表明排油圈直径随发酵液浓度的增加而增大。
|
| 关 键 词： | 生物表面活性剂 检测 排油圈法 |
Improvement and Application of Oil Spreading to Detect Biosurfactant |
| |
| Bi Sining,Wang Yanjie,Zuo Yuhu.Improvement and Application of Oil Spreading to Detect Biosurfactant[J].Journal of Heilongjiang August First Land Reclamation University,2009,21(6):58-60. |
| |
| Authors: | Bi Sining Wang Yanjie Zuo Yuhu |
| |
| Institution: | Bi Sining, Wang Yanjie, Zuo Yuhu (1. College of Agriculture, Heilongjiang Bayi Agricultural University, Daqing 163319; 2. College of Life Science and Technology, Heilongjiang Bayi Agricultural University) |
| |
| Abstract: | The liquid paraffin stained by Sudan red Ⅲwas used to improve the oil spreading in this study. The results by the improved method are same as that by the original one, but the degreasing effects of fermentation broth to liquid paraffin were easily to be observed, and could also be photographed, for there were significant color differences between the water phase and the red oil phase. The method is of importance in application for its simplicity. The degreasing activity of BS1 fermentation broth was studied, and the results showed that diameters of oil spreading were increased as the concentrations of fermentation broth increased. |
| |
| Keywords: | biosurfactant detection oil spreading |
| 本文献已被 维普 万方数据 等数据库收录！ |
|
