| 23份烟草种质遗传多样性的SSR和ISSR标记分析 |
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| 引用本文： | 聂琼,刘仁祥. 23份烟草种质遗传多样性的SSR和ISSR标记分析[J]. 西南农业学报, 2011, 24(1) |
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| 作者姓名： | 聂琼 刘仁祥 |
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| 作者单位： | 1. 贵州大学烟草科学研究中心,贵州,贵阳,550025
2. 贵州省烟草品质研究重点实验室,贵州,贵阳,550025 |
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| 基金项目： | 贵州省十一五重大专项项目[(2006)6026〗; 贵州省自然科学基金项目[(2009)2097]; 贵州省烟草公司项目(2006-03) |
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| 摘 要： | 利用SSR和ISSR标记分析23份烟草种质遗传多样性。结果表明:8个SSR引物和8个ISSR引物分别检测到66个和113个多态性位点。不同烟草种质之间的遗传相异系数在0.3259~0.8588(SSR)或0.1369~0.8161(ISSR)。以SSR、ISSR标记分别聚类以及两种标记混合聚类均在0.63处将23个材料分为4类:2个类群和2个独立个类Coker147、Val16或G140,一类群以红花大金元为基础聚类,另一类群以NC82为基础聚类。两种标记的结果相似,均可用来进行烟草种质资源的遗传多样性分析。
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| 关 键 词： | 烟草 SSR ISSR 聚类分析 |
Genetic Diversity of 23 Tobacco Germplasm Resources by SSR and ISSR Analysis |
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| NIE Qiong,LIU Ren-xiang. Genetic Diversity of 23 Tobacco Germplasm Resources by SSR and ISSR Analysis[J]. Southwest China Journal of Agricultural Sciences, 2011, 24(1) |
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| Authors: | NIE Qiong LIU Ren-xiang |
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| Affiliation: | NIE Qiong1,LIU Ren-xiang2(1.Tobacco Research Center,Guizhou University,Guizhou Guiyang 550025,China,2.Guizhou Key Laboratory of Tobacco Quality,China) |
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| Abstract: | The genetic difference among 23 germplasm resources was analyzed by SSR and ISSR analysis to identify their genetic diversity.The results showed that 66 and 113 polymorphic loci were detected from 8 SSR primers and 8 ISSR primers respectively,the genetic different coefficient between different tobacco germplasm resources was 0.3259-0.8588(SSR) and 0.1369-0.8161(ISSR) separately.The cluster analysis showed that 23 tobacco germplasm resources could be divided into 2 groups(Honghuadajinyuan and NC82) and 2 ind... |
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| Keywords: | Tobacco SSR ISSR Cluster analysis |
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