钙离子胁迫对槲蕨孢子体叶的影响

为解析钙离子胁迫对槲蕨孢子体叶片生长影响的生理机制，以两年生槲蕨孢子体叶为实验材料，观察了钙离子胁迫对孢子体叶的影响。将温室培养的两年生槲蕨植株移栽至直径18.5 cm的花盆中，以蛭石为基质，置于光照培养箱，浇施霍格兰德营养液7d后，分别浇施含有不同钙离子含量的霍格兰德营养液，处理14天，根据槲蕨叶片的形态变化确定处理浓度。取0、600、1200 mmol?L-1钙离子浓度处理的槲蕨叶片测定脯氨酸含量、甜菜碱含量、电导率含量、超氧阴离子含量、过氧化氢含量、抗坏血酸过氧化物酶活性、单脱氢抗坏血酸还原酶活性、脱氢抗坏血酸还原酶活性和谷胱甘肽还原酶活性。结果显示：槲蕨孢子体叶的脯氨酸含量随着钙离子处理浓度的逐渐增加逐渐升高，而甜菜碱含量在1200 mmol?L-1显著升高；电导率、超氧阴离子和过氧化氢的含量随着处理浓度的升高而增加；抗坏血酸过氧化物酶、单脱氢抗坏血酸还原酶、脱氢抗坏血酸还原酶和谷胱甘肽还原酶活性随着处理浓度的增加而增强；表明钙离子浓度在1200 mmol?L-1以下时主要产生渗透胁迫作用。

Effects of Calcium ion stress on sporophyte leaf of Drynaria fortune

In order to analyze the physiological mechanism of calcium ion stress on sporophyte leaf growth of Drynaria fortune, the effect of calcium ion stress on sporophyte leaf growth of two-year-old D. fortune was observed. The two-year-old plants were transplanted into a pot with a diameter of 18.5 cm in greenhouse; vermiculite was used as the substrate, and placed in a light incubator. Hoagland nutrient solution was applied for 7 days, and then Hoagland nutrient solution with different calcium content was applied for 14 days. Take 0, 600, 1200 mmol?L-1 calcium ion concentration treated D. fortune leaf, and the contents of proline and betaine, electrical conductivity, Superoxide anion content, hydrogen peroxide content and ascorbic acid peroxidase activity, single dehydrogenation ascorbic acid reductase activity, dehydrogenation ascorbic acid reductase activity and glutathione reductase activity were determined. The results showed that the content of proline in sporophyte leaves increased with increasing calcium ion concentration, while the content of betaine increased significantly at 1200 mmol?L-1. Electrical conductivity and the content of superoxide anion and hydrogen peroxide increased with the increase of treatment concentration. The activity of ascorbate peroxidase, dehydroascorbate reductase, dehydroascorbate reductase and glutathione reductase increased with the increase of treatment concentration. The results showed that calcium ion concentration below 1200 mmol?L-1 mainly produced osmotic stress.