贺兰山两株大型真菌物种分子鉴定与系统地位分析

采用CTAB 法对采集于贺兰山地区的两株野生大型真菌子实体进行基因组DNA 提取，并利用真菌特有引物ITS1F 和ITS4在PCR 基础上对其rDNA 的ITS 区段进行碱基序列测定并与GenBank 数据库中已知的相关序列进行Blast 比对，结果表明，两株野生大型真菌分别属于鳞伞属Pholiota sp.和球盖菇属Stropharia sp.GenBank 登录号分别为FJ596817.1 和JQ627593.1同时在UNITE Database(s)数据库中采用邻接法对两菌株进行系统发育树的构建及遗传距离的计算，结果表明，一菌株与Pholiota tuberculosa (Pers.) Kuyper & Tjall.-BeukUDB015324另一菌株与Stropharia coronilla (Fr. ex Bull.) Qul. UDB015429 UDB015556）的遗传关系最近遗传距离达到5.0。初步鉴定两株野生大型真菌为小瘤鳞伞和齿环球盖菇。

Molecular identification and analysis of phylogenetic position fortwo wild fruiting body-forming fungi in Helan Mountain

The extraction of genomic DNA of two wild macrofungi obtained from Wulashan was carried out by CTABmethod. Then, on the basis of PCR, ITS regions of DNA of the macrofungi were amplifiedby using the special pairs ofprimers ITS1F/ITS4 and sequenced. The sequences were compared with those registered in GenBank database, results ofwhich showed that two fungi belonged to the genus Pholiota sp. and Strophariaceae sp.. Genbank accession numbers of theITS gene was FJ596817.1 and JQ627593.1. Then the phylogenetic trees were constructed by neighbor-joining method andgenetic distance was analyzed in UNITE Database (s). The results showed that two wild fungi had a high genetic distance,one with Pholiota tuberculosa (Pers.) Kuyper & Tjall.-Beuk (UDB015324), and the other with Stropharia coronilla (Fr. exBull.) Qul.(UDB015429, UDB015556), which was up to 5.0. On the basis of the sequence analysis, two wild fruiting bodyformingfungi were identified as Pholiota tuberculosa and Stropharia coronilla