| 猪肺炎支原体p97 R1区基因和大肠杆菌LTB基因的重组和表达 |
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| 引用本文： | 卢会英,沈青春,宁宜宝. 猪肺炎支原体p97 R1区基因和大肠杆菌LTB基因的重组和表达[J]. 中国兽医杂志, 2010, 46(4) |
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| 作者姓名： | 卢会英 沈青春 宁宜宝 |
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| 作者单位： | 中国兽医药品监察所,农业部兽药创新与生物安全评价重点开放实验室,北京,海淀,100081 |
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| 基金项目： | 国家863计划基金资助 |
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| 摘 要： | 本研究从猪肺炎支原体的主要抗原蛋白及其免疫特点出发,将猪肺炎支原体纤毛粘附决定区R1区基因和具有黏膜免疫佐剂作用的大肠杆菌不耐热肠毒素B亚单位(the B subunit of heat-labile enterotoxin LTB)基因重组表达,并且单独表达了R1区基因。将扩增的目的片段插入到表达载体pET-28a(+)中,分别构建了两个原核表达质粒pET28a(+)-rLTBR1和pET28a(+)-rR1,诱导表达了两个融合蛋白rLTBR1和rR1。对表达的目的蛋白进行了SDS-PAGE和Western blot检测,结果表明,表达的蛋白为特异的蛋白。本试验为进一步的研究rLTBR1融合蛋白在黏膜免疫方面的作用打下了基础。
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| 关 键 词： | 猪肺炎支原体 p97蛋白 |
The recombination and expression of R1 region of Mycoplasma hyopneumoniae p97 adhesin with Escherichia coli heat-labile enterotoxin B subunit |
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| LU Hui-ying,SHEN Qing-chun,NING Yi-bao. The recombination and expression of R1 region of Mycoplasma hyopneumoniae p97 adhesin with Escherichia coli heat-labile enterotoxin B subunit[J]. Chinese Journal of Veterinary Medicine, 2010, 46(4) |
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| Authors: | LU Hui-ying SHEN Qing-chun NING Yi-bao |
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| Affiliation: | LU Hui-ying,SHEN Qing-chun,NING Yi-bao (Key Laboratory of Veterinary Drug Innovation , Bio-safety Evaluation of Ministry of Agriculture,Beijing 100081,China) |
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| Abstract: | The objective of this study was to develop a recombinant subunit vaccine(rLTBR1) containing R1 region of p97 adhesin of Mycoplasma hyopneumoniae(Mhp) and B subunit of the heat-labile enterotoxin of Escherichia coli(LTB).The amplified fragment was inserted into the expression vector pET-28a(+)to express recombinant proteins rLTBR1 and rR1.The immunogenicity was detected by Western blot.The results from this study may be of reference to future research. |
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| Keywords: | R1 LTB |
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