| 响应面法优化三百棒多糖提取工艺 |
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| 引用本文： | 田瑞,夏燕,冯佳,杨年安,向阳,陈安平,袁林.响应面法优化三百棒多糖提取工艺[J].安徽农业科学,2018,46(12):4-6,26. |
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| 作者姓名： | 田瑞 夏燕 冯佳 杨年安 向阳 陈安平 袁林 |
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| 作者单位： | 湖北民族学院风湿性疾病发生与干预湖北省重点实验室,湖北恩施,445000;湖北民族学院风湿性疾病发生与干预湖北省重点实验室,湖北恩施,445000;湖北民族学院风湿性疾病发生与干预湖北省重点实验室,湖北恩施,445000;湖北民族学院风湿性疾病发生与干预湖北省重点实验室,湖北恩施,445000;湖北民族学院风湿性疾病发生与干预湖北省重点实验室,湖北恩施,445000;湖北民族学院风湿性疾病发生与干预湖北省重点实验室,湖北恩施,445000;湖北民族学院风湿性疾病发生与干预湖北省重点实验室,湖北恩施,445000 |
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| 基金项目： | 国家自然科学基金项目(81360672)，湖北省民委项目(HBM W2012027) |
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| 摘 要： | 目的]采用响应面法优化提取三百棒多糖的工艺条件。方法]选择料液比、提取温度、提取时间、提取次数4个因素进行单因素试验,基于单因素试验结果,使用BBD响应面对三百棒多糖的提取工艺进行优化,并建立回归模型。结果]最佳提取工艺条件:料液比1∶15(g∶m L),提取温度93℃,提取时间4 h,提取次数3次,在此条件下,三百棒多糖的提取率为4.75%,与模型的预期值4.99%基本相符。结论]该优化工艺合理可行,具有较高的应用价值。
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| 关 键 词： | 三百棒 多糖 响应面 提取工艺 |
Optimization of Extracting Polysaccharide from Toddalia asiatica Lam.by the Response Surface Method |
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| Abstract: | Objective]Response surface methodology was used to optimize the extraction condition of polysaccharide from Toddalia asiatica Lam..Method]The effects of solid-liquid ratio,extraction temperature,extraction time and extraction times by single factor experiments were studied.Subsequently,based on the four factors,a polysaccharide yield regression model was established using the Box-Behnken design.A regres-sion model equation was fitted.Result]The optimum extractions were solid-liquid ratio of 1:15(g:mL),extraction temperature of 93 ℃,extrac-tion time of 4 h,extraction times of 3.Under the conditions, the maximum experimental polysaccharide yield was 4.75%,which was basically consisitent with the predictied of 4.99%.Conclusion]The optimized technology was feasible,and had high application value. |
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