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| 15个果桑无性系指纹图谱的构建 | |
| 引用本文： | 陈艺,郭军战,李辉,姚忙珍. 15个果桑无性系指纹图谱的构建[J]. 西北林学院学报, 2011, 26(6): 62-65 |
| 作者姓名： | 陈艺 郭军战 李辉 姚忙珍 |
| 作者单位： | 1. 西北农林科技大学林学院,陕西杨陵,712100 2. 户县林业局,陕西户县,710300 3. 渭南市林业技术推广站,陕西渭南,714000 |
| 基金项目： | 西北农林科技大学植物遗传育种专项(A212020503) |
| 摘 要： | 用改良的CTAB法提取15份果桑无性系的基因组DNA,通过RAPD分子标记技术,构建不同果桑无性系的指纹图谱。结果表明:每条引物扩增出8～15条清晰地条带,其中多态性条带数为4～15条,多态性比例为50.0%～100.0%。20条随机引物共得到232条清晰的条带,多态性条带为178条,平均多态性比例为76.7%;利用随机引物OPA-09和OPD-05的扩增产物电泳图为基础构建的指纹图谱可以鉴定出15个果桑无性系。 |
| 关 键 词： | 果桑 无性系 指纹图谱 RAPD |
Fingerprints Construction of 15 Fruit Mulberry (Morus spp.) Clones |
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| CHEN Yi,GUO Jun-zhan,LI Hui,YAO Mang-zhen. Fingerprints Construction of 15 Fruit Mulberry (Morus spp.) Clones[J]. Journal of Northwest Forestry University, 2011, 26(6): 62-65 | |
| Authors: | CHEN Yi GUO Jun-zhan LI Hui YAO Mang-zhen |
| Affiliation: | CHEN Yi1,GUO Jun-zhan1,LI Hui2,YAO Mang-zhen3(1.College of Forestry,Northwes A & F University,Yangling,Shaanxi 712100,China,2.Huxia Forest Bureau,Huxian,Shaanxi 710300,3.Forestry Extension Station,Weinan,Shaanxi 714000,China) |
| Abstract: | Modified CTAB method was adopted to extract total DNA of 15 fruit mulberry(Morus spp.) clones.The RAPD fingerprints of 15 fruit mulberry clones were constructed.The results showed that 8-15 bands were clearly amplified from each primers,of which,4-15 bands were of polymorphism and the percentages of primers with polymorphism were from 50.0% to 100.0%.Totally 232 bands were produced using 20 random primers,in which 178 bands showed polymorphism.Fifteen fruit mulberry clones can be identified by using the fin... |
| Keywords: | fruit mulberry clones fingerprint RAPD |
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