| 花叶姜离体再生体系的建立 |
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| 引用本文： | 陈泽雄,刘奕清,黄登艳.花叶姜离体再生体系的建立[J].北方园艺,2009(8). |
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| 作者姓名： | 陈泽雄 刘奕清 黄登艳 |
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| 作者单位： | 重庆文理学院,花卉研究所,重庆高校园林花卉工程研究中心,重庆,永川,402160 |
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| 基金项目： | 重庆市教委能力创新建设资助项目(GCZX0713) |
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| 摘 要： | 以花叶姜茎块为外植体进行离体培养与植株再生研究。结果表明:花叶姜的启动培养基以改良MS+BA 2.0 mg/L+NAA 0.1 mg/L较适宜,继代增殖以改良MS+BA 1.5 mg/L+NAA 0.2 mg/L为宜,增殖系数可达3.8,生根培养基以1/2MS+NAA 0.1 mg/L+IBA 0.1 mg/L为适,生根率达96%,试管苗移栽成活率达97%以上。
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| 关 键 词： | 花叶姜 离体培养 快速繁殖 植株再生 |
In vitro Culture and Plant Regeneration of Alpinia zerumbet cv. Variegata |
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| CHEN Ze-xiong,LIU Yi-qing,HUANG Deng-yan.In vitro Culture and Plant Regeneration of Alpinia zerumbet cv. Variegata[J].Northern Horticulture,2009(8). |
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| Authors: | CHEN Ze-xiong LIU Yi-qing HUANG Deng-yan |
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| Institution: | Flower Research Institute;Chongqing University of Arts and Science;Garden and Flower Engineering Research Center of Chongqing Colleges;Yongchuan;Chongqing 402160;China |
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| Abstract: | Using Alpinia zerumbet cv.Variegata tuber as explants,in vitro culture and rapid propagation were studied.The results showed that the optimum medium for bud regeneration was MS+BA 2.0 mg/L+NAA 0.1 mg/L,the optimum subculture medium was MS+BA 1.5 mg/L+NAA 0.2 mg/L,on which each explant produced up 3.8 plantlets on the average.The frequency of rooting was over 96% on 1/2MS medium supplemented with 0.1 mg/L NAA and 0.1 mg/L IBA.The survive rate after transplant reached above 97%. |
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| Keywords: | Alpinia zerumbet cv Variegata in vitro culture Rapid propagation Plant regeneration |
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