| Abstract: | Monoclonal antibodies against porcine IgG were produced by fusion and characterized. The supernatants of microtiter wells containing fusion hybrids were first screened with an ELISA using semi-purified porcine IgG as antigen. Hybrids reactive in ELISA were cloned by limiting dilution. Further characterization of the specificity of the monoclonal antibodies was done by a combination of two methods: SDS-PAGE electroimmunoblotting and convection blotting of immunoelectrophoretic patterns (IEP-immunoblotting). Using these techniques, we identified monoclonal antibodies specific for porcine Ig gamma chains. |
