菜用大豆种子劣变与生理生化变化的关系

本文以菜用大豆种子为试材,研究了不同活力种子发生的一些生理生化变化。结果表明,随着种子劣变,细胞膜结构和功能受到损伤,浸泡液电导率增加;当种子丧失生活力后,细胞膜损伤严重,并失去修复能力,内含物大量外渗。高活力种子吸胀葫发过程中呼吸代谢旺盛,而低活力种子呼吸强度较弱。当种子失去生活力后,胚轴中测不出过氧化物酶的活性,胚轴、子叶过氧化物酶同工酶谱带减少,峰高降低;胚轴酯酶同工酶谱带减少,峰高降低,而子叶酯酶同工酶很少发生变化。

RELATIONSHIP BETWEEN SEED DETERIORATION AND PHYSIOLOGICAL AND BIOCHEMICAL CHANGES OF VEGETABLE SAYBEANS

The physiological and biochemcal changes of vegetable soybean (Glycine max L. Merr. )seeds in deterioration were investigated. It was found that as the seeds became deteriorated,the structure and function of cell membrane was damaged. The electrical conductivity of soaking solution increased. When the seeds lost viability ,the damage of the membrane was so serious that it could not be recovered. The leakage from the cells was great. Respiratory rate of high vigor seeds was greater than that of low vigor seeds during germination. As the seeds lost viability .peroxidase activity could not be detected in axis. The bands and peaks of peroxidase isozymes of axis and cotyledons decreased and the bands and peaks of esterase isozymes in axis were reduced also. But they remained unchanged in cotyledons.