| 禽流感病毒H5亚型及H7亚型基因的引物设计与多重RT-PCR扩增 |
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| 引用本文： | 张文慧,郭华,王伟利,刘明,钱爱东.禽流感病毒H5亚型及H7亚型基因的引物设计与多重RT-PCR扩增[J].农业科学与技术,2008,9(1):15-17. |
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| 作者姓名： | 张文慧 郭华 王伟利 刘明 钱爱东 |
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| 作者单位： | [1]吉林农业大学,吉林长春130118 [2]吉林出入境检验检疫局,吉林长春130062 [3]中国农业科学院哈尔滨兽医生物技术国家重点实验室,黑龙江哈尔滨150001 |
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| 基金项目： | 吉林省科技厅资助重大项目 |
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| 摘 要： | RT-PCR作为一种现代分子生物学基因诊断技术，具有高度敏感性和特异性，能在数小时内检出痕量病原，可克服传统的禽流感病毒（AIV）诊断技术病毒分离鉴定试验周期长的缺点，为AIV早期快速诊断提供了敏感、快速、实用的方法。引物设计是保证RT-PCR成功的重要前提。笔者对运用Primer Premier5.0软件设计H5和H7亚型AIV的多重RT-PCR引物进行了探讨。
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| 关 键 词： | 禽流感病毒 RT-PCR 生物学 基因诊断 |
Designing Primers for H5 and H7 Subtypes of Avian Influenza Virus and Multiplex RT-PCR Amplification |
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| ZHANG Wen-hui,GUO Hua,WANG Wei-li,LIU Ming,QIAN Ai-dong.Designing Primers for H5 and H7 Subtypes of Avian Influenza Virus and Multiplex RT-PCR Amplification[J].Agricultural Science ＆ Technology,2008,9(1):15-17. |
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| Authors: | ZHANG Wen-hui GUO Hua WANG Wei-li LIU Ming QIAN Ai-dong |
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| Institution: | ZHANG Wen-hui, GUO Hua, WANG Wei-li, LIU Ming, QIAN Ai-dong (1.Jilin Agricultural University, Changchun 130118;2.Jilin Entry-Exit Inspection and Quarantine Bureau of the People＇s Republic of China, Changchun 130062; 3. National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of CAAS , Harbin 150001) |
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| Abstract: | Objective]The research aimed to design primers that are suitable for detecting H5 and H7 subtypes of avian influenza virus(AIV);Method]DNAStar was used to analyze the homology of the sequences of H5 and H7 subtypes of AIV accessed in GenBank,and design primers(by Primer Premier 5.0) on high homologous region of these sequences,and then amplified by RT-PCR.Result]The multiplex RT-PCR amplification,agarose gel electrophoresis and sequencing results showed that the self-designed primers are successful for detecting AIV.Conclusion]It is feasible to rapidly diagnose AIV through this method. |
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| Keywords: | Avian influenza virus Primer Premier 5 0 DNAStar Multiplex RT-PCR amplification |
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