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Marker-assisted Breeding for Disease Resistance in Potato
Authors:A Carrasco  J E Chauvin  B Trognitz  A Pawlak  O Rubio-Covarruvias  E Zimnoch-Guzowska
Institution:1.APPACALE S.A.,Burgos,Spain;2.UMR ABV,Institut National de Recherche Agronomique (INRA),Ploudaniel,France;3.Biotechnology Department,ARC Seibersdorf Research GmbH,Seibersdorf,Austria;4.Zamarte Breeding Company Ltd.,Kamien Kraj,Poland;5.Programa de Papa,Instituto Nacional de Investigaciones Forestales y Agrícolas (INIFAP),Metepec,México;6.Plant Breeding and Acclimatization Institute (IHAR),Research Centre M?ochów,M?ochów,Poland
Abstract:Sub-project 5 of BIOEXPLOIT aims to design durable disease resistance through marker-assisted breeding by converting existing markers for high-throughput application, developing and validating high-throughput marker technologies and pyramiding major R genes and/or quantitative trait loci into elite material. Activities include (1) the fine mapping of the quantitative trait locus PiXspg which accounts for a large proportion of the variation in late blight resistance, (2) converting SNP-based markers and an AFLP marker to easy-to-use-markers, (3) testing of progenies with combined sources of late blight resistance for presence of R genes and agronomic features, (4) backcrossing new sources of resistance to S. tuberosum and molecular screening of breeding materials with marker GP94 linked with gene Rpi-phu1 conferring late blight resistance, (5) evaluating potato clones with enhanced resistance against Phytophthora infestans under field conditions of Toluca (México), and (6) developing populations and marker-assisted breeding for disease resistance.
Keywords:Durable resistance  Genotyping  High throughput marker technology  Late blight  Phenotyping            R genes            Solanum tuberosum
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