马铃薯茎尖组织培养技术研究 |
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引用本文: | 吴兴泉,陈士华,王朔,杨岫峰. 马铃薯茎尖组织培养技术研究[J]. 安徽农业科学, 2009, 37(3): 1039-1040 |
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作者姓名: | 吴兴泉 陈士华 王朔 杨岫峰 |
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作者单位: | 河南工业大学生物工程学院,河南郑州,450001;河南工业大学生物工程学院,河南郑州,450001;河南工业大学生物工程学院,河南郑州,450001;河南工业大学生物工程学院,河南郑州,450001 |
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基金项目: | 河南省教育厅自然科学基础研究项目,河南工业大学校科研基金 |
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摘 要: | [目的]提高离体马铃薯茎尖组织培养的成苗率和脱毒率。[方法]从马铃薯上取生长健壮的芽,经消毒后在显微镜下切取1.0到1.5min长度的茎尖,接种于诱导分化培养基上,研究了不同浓度的GA3、KT与6-BA和不同激素配比以及不同浓度的基础培养基的诱导效果,探讨了茎尖组织诱导分化的最适培养条件。[结果]低浓度的GA3可促进愈伤组织的形成,过高的GA3浓度会抑制愈伤组织的形成;KT比6BA具有更好的诱导效果;在MS+GA30.1mg/L+NAA0.1mg/L+BBA0.5mg/L培养基中激素配比的诱导效果较好。[结论]MS+0.5mg/LKT+0.2mg/LGA3+0.1mg/LNAA是最为适宜的茎尖组织培养基。在28℃、每天光照16h、光照强度〉2000lx的培养条件下,经7d左右即可得到形态良好愈伤组织,经15d左右即可得到分化良好的试管植株。
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关 键 词: | 马铃薯 茎尖组织 组织培养 |
Research on Tissue Culture Technology for Potato Stem-tip |
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Affiliation: | WU Xing-quan et al (College of Bioengineering, Henan Industrial University, Zbengzhou, Henan 450001 ) |
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Abstract: | [Objective] The study aimed to enhance the seedling rate and virus free rate of stem-tip tissue culture of potato in vitro.[Method] The lusty bud was taken from potato,and the stem-tip with length from 1 to 1.5 mm was cut under microscope after disinfection,and then it was inoculated to the medium for induction and differentiation,the induction effects of GA3,KT and 6-BA with different concn.and the different hormone matching as well as the basic medium with different concn.were studied,and the most proper culture conditions for induction and differentiation of stem-tip tissue was discussed.[Result] The GA3 with low concn.could promote callus formation,the exorbitant GA3 concn.would restrain formation of callus;KT had better induction effect than that of 6-BA.In the medium of MS + GA3 0.1 mg/L + NAA 0.1 mg/L + 6-BA 0.5 mg/L,the induction effect was good for the hormone matching.[Conclusion] The MS + 0.5 mg/L KT + 0.2 mg/L GA3 + 0.1 mg/L NAA was the most suitable medium for stem-tip tissue.Under the culture conditions of light intensity > 2 000 lx,temperature of 28 ℃,illumination of 16 h per day,the callus with good shape would be attained about 7 d later,and the test tube plantlets with good differentiation could be acquired about 15 d later. |
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Keywords: | Potato Stem-tip tissue Tissue culture |
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