| 谷氨酸棒状杆菌乙酸盐代谢PTA、AK、ICL和MS酶活性研究 |
| |
| 引用本文： | 阮红,宣日荣. 谷氨酸棒状杆菌乙酸盐代谢PTA、AK、ICL和MS酶活性研究[J]. 浙江大学学报(农业与生命科学版), 2003, 29(5): 529-533 |
| |
| 作者姓名： | 阮红 宣日荣 |
| |
| 作者单位： | 1. 浙江大学,生物技术系,浙江,杭州,310027
2. 浙江省冶金研究院,浙江,杭州,310007 |
| |
| 基金项目： | 国家教育部留学回国人员启动基金资助项目(108201-G50229). |
| |
| 摘 要： | 从谷氨酸棒状杆菌乙酸盐代谢中涉及的磷酸转乙酰酶PTA、乙酸盐激酶AK、异柠檬酸裂解酶ICL和苹果酸合成酶MS 4种酶入手,建立了一套稳定的酶活性测定方法,并对这些酶在葡萄糖和乙酸盐不同碳源代谢中的酶活性特征进行了比较分析,发现碳代谢中存在葡萄糖效应;乙酸盐对谷氨酸棒状杆菌PTA、AK、ICL和MS酶活性有明显的诱导作用.
|
| 关 键 词： | 谷氨酸棒状杆菌 乙酸盐代谢 磷酸转乙酰酶PTA 乙酸盐激酶AK 异柠檬酸裂解酶ICL 苹果酸合成酶MS |
| 文章编号： | 1008-9209(2003)05-0529-05 |
| 修稿时间： | 2003-07-23 |
Studies on activities of PTA, AK, ICL and MS enzymes involved in the acetate metabolism of Corynebacterium glutamicum |
| |
| RUAN Hong,XUAN Ri-rong. Studies on activities of PTA, AK, ICL and MS enzymes involved in the acetate metabolism of Corynebacterium glutamicum[J]. Journal of Zhejiang University(Agriculture & Life Sciences), 2003, 29(5): 529-533 |
| |
| Authors: | RUAN Hong XUAN Ri-rong |
| |
| Abstract: | The four enzymes of acetate kinase (AK), phosphotransacetylase (PTA), isocitrate lyase (ICL) and malate systhase (MS) which are involved in the acetate metabolism of Corynebacterium glu-tamicum have been developed in their assay methods. The enzyme activities of AK, PTA, ICL and MS are analyzed comparatively while C- glutamicum grows on glucose or/and on acetate. The results showed there was "glucose effect" in carbon metabolism and acetate could significantly promote the induction of specific activities of acetate kinase, phosphotransacetylase, isocitrate lyase and malate systhase. |
| |
| Keywords: | Corynebacterium glutamicum acetate metabolism phosphotransacetylase (PTA) acetatekinase (AK) isocitrate lyase (ICL) malate systhase (MS) |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《浙江大学学报(农业与生命科学版)》浏览原始摘要信息 |
|
点击此处可从《浙江大学学报(农业与生命科学版)》下载免费的PDF全文 |
