鸡不同肠段碱性氨基酸转运载体mRNA表达的差异性研究

为研究肉鸡肠道不同肠段碱性氨基酸转运载体rBAT(系统b0, )、y LAT2(系统y L)、CAT1(系统y )、CAT4(系统y )mRNA表达的差异性,以快大型黄羽肉鸡为动物模型,采集30日龄接近平均体重黄羽肉鸡的十二指肠、空肠、回肠和结直肠样品,采用相对定量RT-PCR方法研究不同肠段rBAT、y LAT2、CAT1、CAT4mRNA表达丰度。结果显示:结直肠rBAT、y LAT2的mRNA表达丰度极显著低于十二指肠、空肠和回肠(P<0.01),其在回肠表达丰度高于空肠、十二指肠,差异不显著(P>0.05)。结直肠CAT1 mRNA表达丰度极显著高于十二指肠、空肠和回肠(P<0.01),回肠极显著高于空肠(P<0.01),高出十二指肠27.9%(P=0.111)。结直肠CAT4 mRNA的表达丰度极显著高于其他各个肠段(P<0.01),十二指肠、空肠、回肠CAT4 mRNA的表达丰度依次降低,但相互之间无显著差异。结果表明,位于肠上皮黏膜细胞顶端的碱性氨基酸转运系统b0, 和基底部位的系统y L转运载体mRNA的表达在肠道中的分布类似,显著区别于系统y 。

Difference of Cationic Amino Acid Transporters mRNA Expression in Different Intestinal Segments of Chicken

The objective of this study was to investigate the difference of mRNA expression of cationic amino acid transporter rBAT(system b0, ),y LAT2(system y L),CAT1(system y )and CAT4(system y ) in different intestinal segments of chicken by relative quantitative RT-PCR.Yellow covered chickens at age of 30 days were used and the different intestinal segments were collected.The result showed: rBAT and y LAT2 mRNA abundance in colorectum were significant lower than that of duodenum,jejunum and ileum(P<0.01).rBAT and y LAT2 mRNA expression in ileum were higher than in duodenum and jejunum,but had no significant difference among them(P>0.05);The expression of CAT1 mRNA in colorectum was very significantly higher than that of duodenum,jejunum and ileum(P<0.01).Expression abundance in ileum was higher than in jejunum significantly(P<0.01) and higher than duodenum by 27.9 %(P=0.111).Same as CAT1 mRNA,the expression of CAT4 mRNA in colorectum was higher than that of duodenum,jejunum and ileum significantly(P<0.01);There was no difference among duodenum,jejunum and ileum(P>0.05).The result indicates that the tissue-specific of transporter mRNA expression of cationic amino acid transporter system b0, and system y LAT2 are similar and discriminated with that of system y .