风箱果叶色变化及呈色机理研究

为研究风箱果叶片不同生长发育过程中叶色变化及呈色机理的问题，以风箱果、‘金叶’风箱果、‘紫叶’风箱果为材料，对其叶片从幼嫩到成熟转变过程5 个生长阶段的叶部特征和叶色变化进行比较研究，并采用徒手切片法观察叶片中色素空间分布。结果表明，随着3 种风箱果的生长，叶片面积均呈逐渐增大的趋势，‘紫叶’风箱果叶面积最大；3 种风箱果叶绿素a 含量、叶绿素b 含量、叶绿素/类胡萝卜素均呈现逐渐升高的趋势，风箱果不同生长阶段叶绿素a 含量、叶绿素b 含量均显著高于‘紫叶’风箱果和‘金叶’风箱果；3 种风箱果叶片色素均主要分布于栅栏组织内，在海绵组织和表皮细胞分布较少。上述结果明确了风箱果叶片在生长发育过程中的叶部特征和叶色表现，为其科学合理应用提供了理论依据。

Leaf Color Change and Coloring Mechanism of Physocarpus amurensis

[OThis paper aims to investigate the leaf color change under different growing stages and coloring mechanism of different Physocarpus varieties. Three Physocarpus amurensis varieties, including P. amurensis, P. amurensis‘Luteus’and P. amurensis‘Diabolo’were used as materials, we investigated the leaf characteristics of five different leaf growth stages from youth to mature. In addition, comparative analysis was conducted on characteristics in terms of leaf color change. We also examined the spatial distribution of anthocyanins glycosides in P. amurensis. The results showed that the leaf areas significantly increased as the plants grew, and P. amurensis‘Diabolo’had the biggest value of leaf area. As the leaf grew, chlorophyll a， chlorophyll b, and the ratio of chlorophyll to carotenoids showed same increasing trend. The contents ofchlorophyll a and chlorophyll b of P. amurensis were extremely higher than those of the other two varieties. The leaf anthocyanins glycosides were mainly distributed in the palisade tissue, while lower in the spongy tissue and surface cells. These results above clearly explained leaf characteristics and performance of color change ofthe three P. amurensis varieties, and provided a theoretical basis for the scientific and reasonable application.