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万寿菊杂交一代遗传多态性的SRAP标记分析
引用本文:张西西,徐进,王涛,董爱香,赵梁军.万寿菊杂交一代遗传多态性的SRAP标记分析[J].园艺学报,2008,35(8):1221-1226.
作者姓名:张西西  徐进  王涛  董爱香  赵梁军
作者单位:(中国农业大学观赏园艺与园林系,北京100094;北京市园林科学研究所,北京100102)
摘    要: 应用SRAP (Sequence-Related Amplified Polymorphism) 分子标记对当前市场上推广的48个万寿菊杂交一代品种进行遗传多态性研究,用60个引物组合进行扩增,从中筛选到20个多态性引物组合,共产生289个多态性条带,平均每个引物组合产生14.45个多态性条带,显示了较高的多态性比率。聚类分析20个引物组合的扩增结果,48份材料分为两大类,Jaccard's相似系数在0.25~0.91之间。

关 键 词:万寿菊  杂交种  多态性  SRAP
收稿时间:2008-03-13

Genetic Diversity with Molecular Marker SRAP of Tagetes erecta L.
ZHANG Xi-xi,XU Jin,WANG Tao,DONG Ai-xiang,ZHAO Liang-jun.Genetic Diversity with Molecular Marker SRAP of Tagetes erecta L.[J].Acta Horticulturae Sinica,2008,35(8):1221-1226.
Authors:ZHANG Xi-xi  XU Jin  WANG Tao  DONG Ai-xiang  ZHAO Liang-jun
Institution:(Department of Ornamental Horticulture and Landscape Architecture, China Agricultural University, Beijing 100094,China; Beijing Institute of Horticulture and Gardening,Beijing 100102,China)
Abstract:Study on genetic diversity of Marigold could benefit the research of cultivar property right protecting,seanged purity testing,and genetic breeding. A new molecular marker system-SRAP (Sequence-Related Amplified Polymorphism) was firstly applied on 48 marigold hybrids genetic diversity research. 20 primer pairs selected from 60 amplified 289 polymorphic bands with an average of 14.45 polymorphic bands perprimer pair. Cluster analysis using UPGMA method based on the data of SRAP amplified bands by 20 primer pairs showed 48 hybrids could be distinguished into two main groups. Jaccard s similarity coefficient ranged from 0.25-0.91.
Keywords:SRAP
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