| 携带绿色荧光蛋白的重组昆虫杆状病毒的构建 |
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| 引用本文： | 于峰,沈卫德,刘丽华,王文兵.携带绿色荧光蛋白的重组昆虫杆状病毒的构建[J].安徽农业大学学报,2005,32(2):240-242. |
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| 作者姓名： | 于峰 沈卫德 刘丽华 王文兵 |
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| 作者单位： | 江苏大学生命科学研究院,镇江,212013;苏州大学农业科学技术学院,苏州,215006 |
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| 基金项目： | 江苏省教育厅科研项目
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江苏大学校科研和教改项目 |
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| 摘 要： | 将GFP cDNA片段克隆到昆虫杆状病毒的转移质粒pBacPAK8上,构建成携带绿色荧光蛋白的表达载体pBacPAK8-GFP;采用脂质体共转染法,将载体pBacPAK8-GFP DNA和亲本病毒Ac-BacPAK6 DNA共转染Sf21细胞,荧光均匀地分布于整个细胞.病毒经空斑分离法,成功分离病毒克隆,获得了重组绿色荧光蛋白的杆状病毒.
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| 关 键 词： | 昆虫 杆状病毒 绿色荧光蛋白 感染 |
| 文章编号： | 1672-352X(2005)02-0240-03 |
| 修稿时间： | 2004年11月17日 |
Construction of Recombinant Baculovirus Carrying Green Fluorescence Protein(GFP) |
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| YU Feng,SHEN Wei-de,LIU Li-hu,WANG Wen-bing.Construction of Recombinant Baculovirus Carrying Green Fluorescence Protein(GFP)[J].Journal of Anhui Agricultural University,2005,32(2):240-242. |
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| Authors: | YU Feng SHEN Wei-de LIU Li-hu WANG Wen-bing |
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| Abstract: | The gfp DNA fragments were amplified and inserted into baculovirus expression vector pBacPAK8. pBacPAK8-GFP DNA and viral Ac-BacPAK6 DNA were cotransfected into Sf21 cells with lipofectin. Observation under fluorescence microscope showed that the green fluorescence was spread in entire Sf21 cells,testifying expression of gfp to be easily detectable. The baculovirus plaques were picked up and the clones of baculovirus were isolated successfully. |
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| Keywords: | baculovirus GFP recombination infection |
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