木薯钾离子通道蛋白MeAKT1的基因克隆及转基因拟南芥构建

钾元素参与植物体多种重要的生理功能。AKT1作为钾离子(K+)通道中的重要组分,介导拟南芥根对钾离子的吸收,然而木薯中的AKT1基因尚未被克隆。为探究木薯AKT1基因的功能,本研究通过qRT-PCR检测低钾处理后木薯中AKT1基因的表达量,发现木薯中AKT1基因的表达量在低钾处理后升高,可知木薯AKT1基因在木薯响应低钾胁迫中发挥作用。通过PCR扩增获得木薯AKT1基因的目的片段,将该片段连接到过表达载体pEGAD中,转化到拟南芥,获得转基因植株,并对木薯AKT1基因的启动子区域进行分析。这将为进一步研究木薯中AKT1基因的生理功能及其在木薯抗低钾胁迫中的作用机制提供帮助。

Gene Cloning of Potassium Channel Protein MeAKT1 in Cassava and Construction of Transgenic Arabidopsis thaliana

Potassium is involved in many important physiological functions of plants.As an important constituent of potassium(K+)channel,AKT1 mediates potassium uptake in Arabidopsis thaliana roots.However,the AKT1 in cassava has not been cloned yet.In this study,to explore the function of cassava AKT1,the expression of AKT1 in cassava after low potassium treatment was detected by qRT-PCR.We found that the expression of AKT1 in cassava increased after low potassium treatment,indicating that cassava AKT1 might plan a role in cassava response to low potassium stress.The coding sequence of cassava AKT1 was amplified by PCR,the fragment was linked to the overexpression vector pEGAD and transformed into Arabidopsis thaliana to obtain transgenic plants.In addition,the promoter region of cassava AKT1 was also analyzed.Our data will be helpful to further study the physiological function of AKT1 in cassava and its mechanism in resisting low potassium stress.