33份“红橘”种质资源的ISSR分析

为分析"红橘"种质资源,利用ISSR分子标记对33份"红橘"种质资源进行遗传多样性分析,利用NTsys 2.10e构建UPGMA树状图,从27条引物中筛选出14条,对33份"红橘"种质资源的DNA进行PCR扩增,得到148条谱带。其中多态性条带120条,多态位点百分率80.53%。33份"红橘"种质资源的遗传相似系数在0.570~0.970之间。以遗传相似系数0.780为阈值,将33份"红橘"种质资源划分为7个组(A,B,C,D,E,F,G)。该研究结果表明33份种质资源遗传差异较大,ISSR能较好地将它们区分开,有助于福橘果实品质改良和福建省红橘种质资源的发展。

The ISSR Analysis of 33"Red Tangerine"Germplasm Resources

To analyze germplasm resources of"red tangerine",genetic diversity of 33"red tangerine"germplasm resources was analyzed using ISSR molecular markers in this study.The UPGMA dendrogram was constructed using NTsys 2.10 e,fourteen of the 27 primers were screened,and DNA of 33"red tangerine"germplasm resources was amplified by PCR,and 148 bands were amplified.Among them,there were 120 polymorphic bands,and the percentage of polymorphic loci was 80.53%.The genetic similarity coefficient of 33"red tangerine"germplasm resources was between 0.570 and 0.970.With the genetic similarity coefficient of 0.780 as the threshold,"red tangerine"germplasm resources were divided into 7 groups(A,B,C,D,E,F,G).The results indicate that the genetic differences between 33 germplasm resources are large,and the ISSR can better distinguish them.The results provided a reference for the improvement of the quality of Fuju and the development of red tangerine germplasm resources in Fujian Province.