2种甜玉米自交系幼胚再生体系的建立

本试验研究不同基因型、幼胚大小、培养基类型及培养基添加物对2种甜玉米(2-105和2-118)自交系幼胚愈伤及胚性愈伤组织诱导率的影响。结果表明,基因型、培养基类型、幼胚大小及不同浓度脯氨酸和2,4-D处理对愈伤及胚性愈伤诱导率影响差异均显著。2种基因型最适宜的基本培养基为N6培养基,且2-118的愈伤及胚性愈伤诱导率显著高于2-105。幼胚在1.5~2.0 mm时,2-105和2-118的愈伤及胚性愈伤诱导率均最高,分别在77%和51%以上。2-105和2-118在脯氨酸浓度分别为650 mg/L、750 mg/L时幼胚的愈伤及胚性愈伤诱导率最高,且显著高于其它2个水平。2种基因型在2.5 mg/L的2,4-D诱导下愈伤及胚性愈伤诱导率均最高,且均显著高于其它2个水平。本研究获得了2种甜玉米自交系的再生体系,为甜玉米遗传转化和分子育种提供基础。

Establishment of Sweet Maize Immature Embryo Regeneration System for Two Inbred Lines

In this study, the effects of different genotypes, immature embryos size, medium types, medium additions on induction rate of callus and embryogenic callus in sweet maize for 2 inbred lines were investigated.The results showed that the genotypes, medium types, immature embryos size and different treatments of proline and 2,4-D had significant effects on the induction rate of callus and embryogenic callus(p<0.05). The induction rates of callus and embryogenic callus from 2-118 were significantly higher than that from 2-105, and the best basal medium for the 2 genotypes was N6 medium. Moreover, the induction rates of callus and embryogenic callus both from the 2-105 and 2-118 were highest when the immature embryos size were 1.5~2.0 mm, more than 77%and 85%, respectively. Besides, the induction rates of callus and embryogenic callus both from the 2-105 and2-118 were highest when the concentrations of the proline were 650 mg/L and 750 mg/L separately and the concentrations of the 2,4-D were 2.5 mg/L. Regeneration system of 2 inbred lines were obtained in this study which would provide important foundation for genetic transformation and molecular breeding of sweet maize.