普通烟草叶柄性状的遗传分析和基因初定位

烟草叶柄位于叶片的基部,是叶片的重要组成部分。在普通烟草种质资源中,普遍存在有无叶柄两种类型。本研究以无叶柄的烤烟品种‘K326’和有叶柄的晒晾烟种质‘枯斑三生’为亲本,杂交获得F1,进而构建F2、BC1F1和BC2F1群体,利用烟草‘430K’SNP芯片对147个BC2F1个体进行基因型分析,开展烟草叶柄有无性状的遗传分析和定位。结果表明,普通烟草有叶柄性状受一对显性基因控制。初步将控制烟草叶柄有无的基因TP1定位在烟草第5号连锁群上,与标记M5-83共分离。研究结果为进一步精细定位和克隆烟草叶柄相关基因,揭示烟草叶柄遗传机制和发育机理提供了依据。

Genetic Analysis and Gene Mapping of Petiole Traits in Common Tobacco

Tobacco petiole is an important part of leaf,which is located at the base of leaf.There are two types of with or without petiole in common tobacco germplasm resources.F1 was obtained by a cross between a flue-cured tobacco cultivar'K326'without petiole and a sun-cured tobacco cultivar N.tabacum var.Samsun NN with petiole.Furthermore,F2,BC1F1 and BC2F1 populations were obtained by self-cross of F1 and backcross and 147 BC2F1 individuals was genotyped using tobacco'430K'SNP chip.The results showed that the petiole trait was controlled by a pair of dominant major genes.The gene TP1 which controls petiole trait in tobacco was mapped on chromosome 5 in tobacco,and was Co-segregated from the marker M5-83.This results lay a foundation for further fine localization and cloning genes related to tobacco petiole and for revealing the genetic and developmental mechanisms of tobacco petiole.