蚯蚓MT基因植物表达载体的构建及烟草转化

为了提高植物对重金属的耐受力和富集能力,从而减少重金属对土壤的污染,本研究将克隆的赤子爱胜蚓(Eiseniafoetia)MT基因,装入植物表达载体pPZP111,构建了植物表达载体pPZP-efMT。然后用三亲法将其导入根瘤农杆菌EHA105中,通过叶盘法转化烟草。经PCR检测,efMT基因已整合到烟草基因组中,为进一步验证转基因烟草的抗重金属能力奠定了基础。

Construction of Plant Express Vector of Metallothionein Gene and Transform Tobacco

In order to improve the plants,capabilities of tolerance and heavy metals of enrichment,eventually reduce the pollution of soil,the Eisenia foetida MT gene by PCR and successfully constructed the express vector pPZP-efMT were cloned.The recombined vector was transformed into the EHA105 by triprental cross,and then was transformed tobacco by leaf-disc method.Transgenic tobaccos were identified by PCR.All of this process would make the foundation of plant anti-metals.