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| 虫草制品中核苷类有效成分分析方法研究 | |
| 引用本文： | 陈伟,黄开华,付婷,周庆林,高新华,张红霞.虫草制品中核苷类有效成分分析方法研究[J].天津农业科学,2011,17(1):37-39. |
| 作者姓名： | 陈伟 黄开华 付婷 周庆林 高新华 张红霞 |
| 作者单位： | 1. 上海农业科学院植物保护研究所,上海,201106 2. 沈阳农业大学分析测试中心,辽宁沈阳,101161 3. 阜新市林业技术推广站,辽宁阜新,123000 |
| 基金项目： | 上海市科委国内合作项目 |
| 摘 要： | 采用HPLC法建立测定虫草中11种有效成分的分析方法。采用碳十八色谱柱,甲醇和磷酸盐水溶液进行梯度洗脱,11种有效成分分别为:胞嘧啶、尿嘧啶、次黄嘌呤、尿苷、肌苷、胸腺嘧啶、腺嘌呤、鸟苷、2'-脱氧胸苷、腺苷、虫草素。考察了方法的线性范围、稳定性以及仪器的精密度。试验数据显示上述指标均在可接受的范围内。 |
| 关 键 词： | 反相高效液相色谱法 冬虫夏草 北冬虫夏草 金水宝胶囊 宁心宝胶囊 核苷类物质 |
Research on Analytic Methods of Nucleotides in Cordyceps Preparation |
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| CHEN Wei,HUANG Kai-hua,FU Ting,ZHOU Qing-lin,GAO Xin-hua,ZHANG Hong-xia.Research on Analytic Methods of Nucleotides in Cordyceps Preparation[J].Tianjin Agricultural Sciences,2011,17(1):37-39. | |
| Authors: | CHEN Wei HUANG Kai-hua FU Ting ZHOU Qing-lin GAO Xin-hua ZHANG Hong-xia |
| Institution: | CHEN Wei~1,HUANG Kai-hua~1,FU Ting~2,ZHOU Qing-lin~3,GAO Xin-hua~1,ZHANG Hong-xia~1 (1.Plant Protection Research Institute,Shanghai Academy of Agricultural Sciences,Shanghai 201106,China,2.Analysis and Testing Center,Shenyang Agricultural University,Shenyang,Liaoning 110161,3.Fuxin Forest Technology Extension Station,Fuxin,Liaoning 123000,China) |
| Abstract: | A high performance liquid chromatographic(HPLC) method was developed for the determination of nucleotides.Meanwhile, a comparative analysis of nucleotides in Cordyceps preparation was conducted by the developed method.Results showed that a good separation of Cytosine,Uracil,Hypoxanthine,Uridine,Thymine,Adenine,Inosine,Guanidine,2'-thymidine,Adenosine, cordycepin were achieved.This method was stable,precise and reproducible and was successfully applied to the determination of nucleosides in cordyceps prepara... |
| Keywords: | HPLC Cordyceps sinensis Cordyceps militaris Jinshuibao capsule Ningxinbao capsule nucleotides |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |