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| 上海牡丹根际土壤DNA提取方法研究 | |
| 引用本文： | 刘志刚,范丙友,史国安.上海牡丹根际土壤DNA提取方法研究[J].天津农业科学,2011,17(1):84-87. |
| 作者姓名： | 刘志刚 范丙友 史国安 |
| 作者单位： | 1. 上海植物园,上海,200231;河南科技大学,农学院,河南,洛阳,471003 2. 河南科技大学,农学院,河南,洛阳,471003 3. 上海植物园,上海,200231 |
| 基金项目： | 国家自然科学基金，国家林业局林业公益性行业科研专项，上海市重点科技攻关项目，上海市农委科技攻关项目，上海市绿化和市容管理局科技攻关项目，河南科技大学人才基金 |
| 摘 要： | 采用玻璃珠-溶菌酶法、蛋白酶K-SDS-热处理法、PVPP-溶菌酶-SDS法等3种土壤DNA提取方法,分别提取上海地区牡丹根际土壤DNA。玻璃珠-溶菌酶法、蛋白酶K-SDS-热处理法提取的DNA,含有大量的杂质,抑制PCR扩增反应,但分别用DNA溶液过柱纯化后不同倍数稀释和凝胶电泳回收纯化,都能扩增出相应的目的条带。PVPP-溶菌酶-SDS法提取的DNA杂质较少,直接用于PCR扩增反应,可以扩增出与预期一致的条带。PVPP-溶菌酶-SDS法不需要纯化,时间较短,可以有效地扩增出预期条带,适合上海地区牡丹根际土壤DNA的提取。 |
| 关 键 词： | 牡丹 根际土壤 DNA提取 PCR |
Study on DNA Extraction Method in Rhizosphere Soil of Poeny in Shanghai |
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| LIU Zhi-gang,FAN Bing-you,SHI Guo-an.Study on DNA Extraction Method in Rhizosphere Soil of Poeny in Shanghai[J].Tianjin Agricultural Sciences,2011,17(1):84-87. | |
| Authors: | LIU Zhi-gang FAN Bing-you SHI Guo-an |
| Institution: | LIU Zhi-gang~(1,2),FAN Bing-you~2,SHI Guo-an~1 (1.Shanghai Botanical Garden,Shanghai 200231,China,2.Agricultural College,Henan University of Science and Technology,Luoyang,Henan 471003,China) |
| Abstract: | Three DNA extraction methods of glass bean-lysozyme method,proteinase K-SDS-heating method,PVPP-lysozyme-SDS method were used to extract DNA in rhizosphere soil of poeny in Shanghai.The soil DNA extracted by glass bean-lysozyme method and proteinase K-SDS-heating method included many impurities,which inhibited PCR amplification.After purified by agarose method,the soil DNA was used for PCR amplification.DNA extracted by method of PVPP-lysozyme-SDS included less impurities, which could be used for PCR amplif... |
| Keywords: | PCR |
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