Significance of explant preparation and sizing in Aloe vera L.—A highly efficient method for in vitro multiple shoot induction |
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Authors: | Balraj Singh Neelu Sood |
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Affiliation: | Department of Biotechnology, Guru Nanak Dev University, Amritsar 143005, India |
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Abstract: | The present study was carried out to assess the effect of explant preparation and sizing for in vitro micropropagation of Aloe vera L. The stem nodal explants and shoot tips were cultured on modified Murashige and Skoog's medium (1962) supplemented with different concentrations of 6-benzylaminopurine (BA), kinetin (KIN), indole-3-acetic acid (IAA), indole-3-butyric acid (IBA) and α-naphthaleneacetic acid (NAA) either singly or in combination. The best media composition was found to be MS medium supplemented with IAA (11.42 μM), IBA (9.8 μM) and BA (8.88 μM). The explants were divided into 2 sets, with and without ensheathing leaf base. Explant sizing, pruning and retention of mother tissue was highly significant in induction of multiple shoots and roots. The stem nodal explants with leaf base performed much better than those without such covering. A very high number of shoots and roots grew from these explants. The rooted plantlets were successfully acclimatized and transferred to the green house conditions and finally to field conditions. |
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Keywords: | BA, benzylaminopurine KIN, kinetin IAA, indole-3-acetic acid IBA, indole-3-butyric acid 2,4-D, 2,4-dichlorophenoxyacetic acid NAA, α-naphthaleneacetic acid MS, Murashige and Skoog |
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