Mass propagation of Satyrium nepalense D.Don.—A medicinal orchid via seed culture |
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Authors: | G. Mahendran V. Narmatha Bai |
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Affiliation: | School of Life Sciences, Department of Botany, Bharathiar University, Coimbatore 641046, Tamil Nadu, India |
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Abstract: | An in vitro plant regeneration protocol was successfully established in Satyrium nepalense, a terrestrial orchid by culturing immature seeds from unripe fruits. Seeds were germinated on Murashige and Skoog, Knudson C and Knudson C modified Morel medium. The germination of the seeds and development of protocorm was highest in MS medium (86.7%) followed by Knudson C modified Morel medium (74%) and Knudson C medium (61.2%). Among the cytokinins tried for multiple shoot induction from the protocorm, 1-phenyl-3-(1,2,3-thiadiazol-5-yl)-urea (TDZ) was found to be superior. Indole-3-butyric acid (IBA) was effective for inducing healthy roots. Well-developed plantlets were hardened in vermicompost (leaf litter + cow dung 1:1), sand and coconut husk (1:1:1). |
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Keywords: | Asymbiotic seed germination Protocorm Seed culture Multiple shoots |
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