Identification of gentian cultivars using SCAR markers based on intron-length polymorphisms of flavonoid biosynthetic genes |
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Authors: | Norimoto Shimada Takashi Nakatsuka Yuki Nakano Yuko Kakizaki Yoshiko Abe Takashi Hikage Masahiro Nishihara |
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Institution: | 1. Iwate Biotechnology Research Center, Kitakami, Iwate 024-0003, Japan;2. Hachimantai City Floricultural Research and Development Center, Hachimantai, Iwate 028-7592, Japan |
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Abstract: | Gentian is one of the most important ornamental flowers in Japan. Gentiana triflora, G. scabra and their interspecific hybrids are the main breeding materials. Gentian cultivars are easily proliferated vegetatively, therefore it is important to develop a reliable discrimination method to prevent the illegal propagation and distribution of various high-value cultivars. Here, we report five sequence characterized amplified region (SCAR) markers based on the length polymorphisms in introns of four gentian flavonoid biosynthetic genes. These SCAR markers effectively discriminated nine gentian cultivars and nine breeding lines. This method could be applied in identifying gentian cultivars/lines and therefore will aid in protecting breeders’ rights. |
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Keywords: | Cultivar discrimination DNA marker Flavonoid biosynthetic gene Gentian SCAR |
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