| 鸡胚胎干细胞的分离培养与鉴定 |
| |
| 引用本文： | 李欣,于永生,吕阳,柳俭强,金海国,朴庆林,赵中利.鸡胚胎干细胞的分离培养与鉴定[J].中国兽医学报,2020(6):1228-1231. |
| |
| 作者姓名： | 李欣 于永生 吕阳 柳俭强 金海国 朴庆林 赵中利 |
| |
| 作者单位： | 吉林省农业科学院畜牧科学分院;西北农林科技大学动物科技学院 |
| |
| 基金项目： | 吉林省农业科学院创新工程资助项目(CXGC-2018DC001)。 |
| |
| 摘 要： | 利用胰蛋白酶消化法从X期鸡胚中分离胚胎干细胞(embryonic stem cells,ESCs),以鸡胚成纤维细胞为滋养层,进行体外培养,采用形态法和标志分子检测对获得的干细胞进行鉴定。结果显示,获得典型的呈巢状或岛状的鸡胚胎干细胞(chicken embryonic stem cells,cESCs)克隆,细胞碱性磷酸酶(alkaline phosphatase,AKP)染色呈蓝紫色,表明具有较高的内源性AKP活性;胚胎阶段特异性表面抗原SSEA-1(stage specific embryonic antigen 1,SSEA-1)鉴定结果呈阳性,显示cESCs克隆具有多能性。本试验成功分离出cESCs,可体外培养并保持未分化状态及多能性。
|
| 关 键 词： | 鸡 胚胎干细胞 体外培养 碱性磷酸酶 胚胎阶段特异性表面抗原 |
Isolation,culture and identification of chicken embryonic stem cells |
| |
| LI Xin,YU Yong-sheng,LYU Yang,LIU Jian-qiang,JIN Hai-guo,PIAO Qing-lin,ZHAO Zhong-li.Isolation,culture and identification of chicken embryonic stem cells[J].Chinese Journal of Veterinary Science,2020(6):1228-1231. |
| |
| Authors: | LI Xin YU Yong-sheng LYU Yang LIU Jian-qiang JIN Hai-guo PIAO Qing-lin ZHAO Zhong-li |
| |
| Institution: | (Branch of Animal Husbandry,Jilin Academy of Agricultural Sciences,Gongzhuling City,Jilin 136100,China;College of Animal Science and Technology,Northwest A&F University,Yangling,Shaanxi 712100,China) |
| |
| Abstract: | Embryonic stem cells(ESCs)were isolated from stage X chicken embryo by trypsin digestion,and cultured in vitro with chicken embryo fibroblasts as trophoblast,and the stem cells obtained were identified by morphological method and marker molecular detection.The results showed that typical nests or islands of chicken embryonic stem cells were obtained,and the cell alkaline phosphatase(AKP)stain was bluish-purple,indicating a high endogenous AKP activity.The result of embryonic SSEA-1 is positive,showing that the cloning of cESCs is pluripotent.This experiment successfully isolated chicken embryonic stem cells,which can be cultured in vitro and remain undifferentiated and pluripotent. |
| |
| Keywords: | chicken ESCs in vitro culture AKP SSEA-1 |
| 本文献已被 CNKI 维普 等数据库收录！ |
|
