| 黑皮鸡址从菌固体培养基及温度优化 |
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| 引用本文： | 王皓,刘迪,李官浩,陈功鑫,于婷婷,金香花.黑皮鸡址从菌固体培养基及温度优化[J].中国食用菌,2020(2):25-28. |
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| 作者姓名： | 王皓 刘迪 李官浩 陈功鑫 于婷婷 金香花 |
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| 作者单位： | 延边大学农学院;延边大学农学院食（药）用真菌研究所;延边大学食品研究中心;延边百草药材有限公司 |
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| 基金项目： | 延边大学校企合作项目(延大科合字[2017] 4号);吉林省教育厅"十三五"科学技术项目(JJKH20170462KJ);吉林省科技厅自然科学基金(20170101022JC). |
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| 摘 要： | 为研究不同碳源、氮源、无机盐以及温度对黑皮鸡地从菌生长的影响,通过单因素分析筛选出最适合黑皮鸡地从菌生长的培养基及培养温度。以菌丝干重为指标,通过3因素3水平正交试验,得出最佳固体培养基配方为琼脂20 g·L^-1、果糖25 g·L^-1、酵母浸膏2.5 g·L^-1、硫酸钾0.7 g·L^-1,最适培养温度为25℃。
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| 关 键 词： | 黑皮鸡地从菌 固体培养基 温度 菌丝干重 |
Optimization of Solid Medium and Temperature of Oudemansiella radicata |
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| WANG Hao,LIU Di,LI Guan-hao,CHEN Gong-xin,YU Ting-ting,JIN Xiang-hua.Optimization of Solid Medium and Temperature of Oudemansiella radicata[J].Edible Fungi of China,2020(2):25-28. |
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| Authors: | WANG Hao LIU Di LI Guan-hao CHEN Gong-xin YU Ting-ting JIN Xiang-hua |
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| Institution: | (Agricultural College,Yanbian University,Yanji 133002,China;Institute of Edible and Medicinal Fungi,Agricultural College,Yanbian University,Yanji 133002,China;Institute of Food Research Center,Yanbian University,Yanji 133002,China;Yanbian Baicao Medicinal Materials Co.Ltd.,Huichun 133300,China) |
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| Abstract: | The effects of different carbon sources, nitrogen sources and inorganic salts on the growth of Oudemansiella radicata were studied, and the optimum conditions were selected to optimize the culture formula. Taking the mycelium dry weight as the index, the optimum solid medium formula was obtained by three factors and three levels orthogonal test. The best formula is agar20 g·L^-1, fructose 25 g·L^-1, yeast extract 2.5 g·L^-1, potassium sulfate 0.7 g·L^-1, and the optimum temperature is 25℃. |
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| Keywords: | Oudemansiella solid medium temperature mycelium dry weight |
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