芹菜软腐病拮抗芽胞杆菌筛选及防治效果

本研究从芹菜根际土壤分离筛选得到一株对芹菜软腐病具有良好防治效果的菌株ZF75。通过菌落形态观察、生理生化特性及16S rDNA序列分析,初步将该菌株鉴定为解淀粉芽胞杆菌Bacillus amyloliquefaciens。平板对峙结果表明,菌株ZF75对番茄疮痂病菌等10种病原菌具有较强的拮抗效果。用温室栽培试验对菌株ZF75进行防病效果初探,试验结果表明,菌株ZF75对芹菜软腐病防效可达89.62%。同时,该菌株对芹菜具有显著的促生效果,经菌株ZF75菌悬液处理后的芹菜平均株高和茎粗分别达24.18 cm和0.51 cm。综合分析表明,菌株ZF75具有良好的田间应用开发潜力。

Screening and Biocontrol of Antagonistic Bacillus against Celery Soft Rot

A biocontrol bacterium ZF75 isolated from the rhizosphere soil of celery had significant inhibitory effecton Pectobacterium carotovorum subsp. odoriferum. Based on biological characteristics, physiological and biochemical characteristics, and multilocus sequence analysis, the strain ZF75 was identified as Bacillus amyloliquefaciens. Plate confrontation assay demonstrated that strain ZF75 has strong inhibitory effects against ten pathogens such as Xanthomonas campestris pv. vesicatoria. Potted plant test in greenhouse indicated that strain ZF75 significantly reduced disease incidence of celery bacterial soft rot, with the control efficacy of 89.62%. Meanwhile, strain ZF75 could significantly promote the plant growth, drenching treatment with strain ZF75 increased plant height and stem diameter by 24.18 cm and 0.51 cm, respectively. In summary, the strain ZF75 is a potential biocontrol agent to be applied in the field.