| 氮离子束对离体和体内DNA影响的初步研究 |
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| 引用本文： | 鲁润龙,朱学良,余增亮,邓建国. 氮离子束对离体和体内DNA影响的初步研究[J]. 安徽农业大学学报, 1991, 0(4) |
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| 作者姓名： | 鲁润龙 朱学良 余增亮 邓建国 |
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| 作者单位： | 中国科学技术大学生物系 合肥(鲁润龙，朱学良)，中国科学院等离子体研究所 合肥(余增亮)，中国科学院等离子体研究所 合肥(邓建国) |
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| 摘 要： | 本文研究了N~+离子束对体内(JM109)和体外质粒PBR322DNA的辐照效应,经琼脂糖电泳检测发现:在体外直接辐照,引起超螺旋环状DNA的单链断裂而转变成开环状DNA:而体内质粒PBR322DNA由于质膜和细胞壁的保护,或命中概率降低,尚存在微弱的超螺旋DNA带,此说明,N~+离子束作用环状DNA能引起DNA链的断裂,这具有生物学的意义。
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| 关 键 词： | 离子束 诱变 DNA |
PRELIMINARY STUDY ON EFFECTS OP NITROGEN CATION BEAM ON IN VITRO AND IN VIVO DNAS |
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| Lu Runlong,Zhu Xueliang,Yu Zhenliang,Den Jianguo Department of Biology USTC Institute of Plasm,Academia Sinica Hefei. PRELIMINARY STUDY ON EFFECTS OP NITROGEN CATION BEAM ON IN VITRO AND IN VIVO DNAS[J]. Journal of Anhui Agricultural University, 1991, 0(4) |
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| Authors: | Lu Runlong Zhu Xueliang Yu Zhenliang Den Jianguo Department of Biology USTC Institute of Plasm Academia Sinica Hefei |
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| Affiliation: | Lu Runlong,Zhu Xueliang,Yu Zhenliang,Den Jianguo Department of Biology USTC Institute of Plasma,Academia Sinica Hefei |
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| Abstract: | In this paper,we discussed the effects of Nitrogen cation beam on in vitroplasmid DNA is discussed.Agarose gel electerophoresis shows that, afterirradiation superhelical circular DNA becomes nicked circular DNA. Thissuggests that nitrogen cation beam can break circular DNA and thus hasbiological effects. |
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| Keywords: | Cation beam Mutation DNA |
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