Possible involvement of fructose 1,6-bisphosphatase in cold-induced sweetening of potato tubers |
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Authors: | Pieternel A. M. Claassen Miriam A. W. Budde |
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Affiliation: | (1) ATO-DLO Agrotechnological Research Institute, P.O Box, 17,6700 AA Wageningen, The Netherlands |
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Abstract: | Summary In measuring fructose 1,6-bisphosphatase (EC 3.1.3.11; FBPase) in potato tubers, we used anti PFP antibodies to ensure that the assay was specific for FBPase and that PPi:fructose 6-phosphate 1-phosphotransferase (EC 2.7.1.90: PFP) did not contribute to the production of fructose 6-phosphate (Fru-6-P). The involvement of cytosolic FBPase in cold sweetening was determined by monitoring enzyme activity during storage ofSolanum tuberosum cv. Erntestolz tubers at 2 or 8°C. In contrast to the rapid increase in sucrose and reducing sugars, the specific activity of FBPase showed no change and PFP rose slightly during storage at 2°C. Sugar concentrations and enzyme activities remained virtually unchanged during storage at 8°C. These data allow the interconversion of fructose 1.6-bisphosphate (Fru-1,6-P2) to Fru-6-P and vice versa by FBPase and PFP, respectively, in potato tubers, but do not support a causal relationship between coarse metabolic control of FBPase and cold-induced sweetening. |
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Keywords: | FBPase PFP Solanum tuberosum L. low temperature storage sugars |
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