| Experiment on Tissue Culture Technique of Saposhnikovia divaricata （Turcz.） Schischk |
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| 作者姓名： | TONG Weishuang FAN Ruifeng GUO Shicheng CHANG Ying |
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| 作者单位： | TONG Weishuang,GUO Shicheng,CHANG Ying(College of Life Sciences, Northeast Agricultural University, Harbin 150030, China);FAN Ruifeng(College of Pharmacy, Heilongjiang University of Chinese Medicine, Harbin 150040, China)
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| 基金项目： | 黑龙江省自然科学基金 |
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| 摘 要： | The study aimed to optimize the induction and differentiation medium by exploreing different tissue culture of Saposhnikovia divaricata （Turcz.） Schischk. In tissue culture with the root, stem segments, young leaf, cotyledonary node and axillary bud of Saposhnikovia divaricata （Turcz.） Schischk as explants, a lot of plantleles were obtained and the corresponding plant regeneration-system was established. The results showed that when use MS＋1.0 mg·L^-1 6-BA＋0.2 mg·L^-1 NAA as callus induction medium, the cotyledonary node had the highest bourgeon rate, and its callus was better than any others; MS＋2 mg·L^-1 6-BA＋0.4 mg·L^-1 NAA was the best adventitious buds induction medium, and the best adventitious buds induced condition was 3% sucrose as carbon source, illumination for 12-14 h·d^-1 and pH 5.8, The best rootage medium was 1/2 MS＋0.5 mg·L^-1 NAA.
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| 关 键 词： | 组织培养技术 防风 诱导培养基 实验 植株再生系统 愈伤组织 生根培养基 NAA |
Experiment on Tissue Culture Technique of Saposhnikovia divaricata(Turcz.) Schischk |
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| TONG Weishuang,FAN Ruifeng,GUO Shicheng,CHANG Ying.Experiment on Tissue Culture Technique of Saposhnikovia divaricata(Turcz.) Schischk[J].Journal of Northeast Agricultural University,2009,16(1):19-24. |
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| Authors: | TONG Weishuang FAN Ruifeng GUO Shicheng CHANG Ying |
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| Institution: | 1. College of Life Sciences, Northeast Agricultural University, Harbin 150030, China
2. College of Pharmacy, Heilongjiang University of Chinese Medicine, Harbin 150040, China |
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| Abstract: | The study aimed to optimize the induction and differentiation medium by exploreing different tissue culture of Saposhnikovia divaricata (Turcz.) Schischk. In tissue culture with the root, stem segments, young leaf, cotyledonary node and axillary bud of Saposhnikovia divaricata (Turcz.) Schischk as explants, a lot of plantleles were obtained and the corresponding plant regeneration-system was established. The results showed that when use MS+1.0 mg·L-1 6-BA +0.2 mg·L-1 NAA as callus induction medium, the cotyledonary node had the highest bourgeon rate, and its callus was better than any others; MS +2 mg·L-1 6-BA +0.4 mg·L-1NAA was the best adventitious buds induction medium, and the best adventitious buds induced condition was 3% sucrose as carbon source, illumination for 12-14 h·d-1 and pH 5.8. The best rootage medium was 1/2 MS+0.5 mg·L-1 NAA. |
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| Keywords: | Saposhnikovia divaricata (Turcz ) Schischk tissue culture callus plant regeneration |
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