| 孝顺竹种胚愈伤组织悬浮培养条件优化 |
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| 引用本文： | 袁金玲,张朵,顾小平,岳晋军,陈益泰.孝顺竹种胚愈伤组织悬浮培养条件优化[J].分子植物育种,2009,7(4):839-844. |
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| 作者姓名： | 袁金玲 张朵 顾小平 岳晋军 陈益泰 |
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| 作者单位： | 1. 中国林业科学研究院亚热带林业研究所,富阳,311400
2. 中国林业科学研究院亚热带林业研究所,富阳,311400;河南农业大学林学院,郑州,450002 |
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| 基金项目： | 浙江省重大科技专项重点项目和中国林科院亚热带林业研究所基本科研业务费 |
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| 摘 要： | 本研究利用孝顺竹种胚诱导出的淡黄色胚性愈伤组织为材料,对影响细胞悬浮培养的摇床转速、培养液2,4-D浓度、继代天数、接种细胞浓度(W/V)等因素进行了研究.结果表明,悬浮培养时的摇床转速以120 r/min时培养效果较好,转速低于100 r/min时,愈伤组织容易结团,分散性差;适宜的2,4-D浓度为4～6 mg/L,低于4 mg/L时愈伤组织易褐变;接种后培养7 d是最佳的转接时期,至多不能超过10 d;接种细胞密度以4.0%最为合适,可保持较高的增殖速度,同时培养产物较多.在50 mL培养液(NB+500 mg/L Pro-line+500 mg/L Glutamine+300 mg/L Hydrolyzed casein+30 mg/L Sucrose+4～6 mg/L 2,4-D)中接种4.0%的愈伤组织,于120 r/min的转速下经过7 d培养,可增殖一倍以上,以后每7 d继代转接一次,培养大约一个月,便可以建立生长迅速、分散性好、均一的悬浮细胞系.
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| 关 键 词： | 孝顺竹 愈伤组织 悬浮培养 转速 |
Suspension Culture Optimization of Seed Embryo Callus from Bambusa multiplex |
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| Yuan Jinling,Zhang Duo,Gu Xiaoping,Yue Jinjun,Chen Yitai.Suspension Culture Optimization of Seed Embryo Callus from Bambusa multiplex[J].Molecular Plant Breeding,2009,7(4):839-844. |
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| Authors: | Yuan Jinling Zhang Duo Gu Xiaoping Yue Jinjun Chen Yitai |
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| Institution: | Yuan Jinling 1 Zhang Duo 1,2 Gu Xiaoping 1 Yue Jinjun 1 Chen Yitai 1 1 Research Institute of Subtropical Forestry,Chinese Academy of Forestry,Fuyang,311400,2 College of Forestry,Henan Agricultural University,Zhengzhou,450002 |
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| Abstract: | Yellowish embryogenic callus induced from mature seed embryo of Bambusa multiplex was used as material. Rotation speed, concentration of 2,4-dichlorophenoxyacetic acid (2,4-D), days of subculture, and inoculation cell density were optimized in this research. The results showed that a rotation speed of 120 r/min was good, callus blocks cling at a rotation speed of less than 100 r/min. The optimal 2,4-D concentration was 4~6 mg/L for the suspension culture system, otherwise browning happened at a concentratio... |
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| Keywords: | 2 4-D |
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