温州水牛遗传多样性的ISSR-PCR分析

为研究浙江省温州水牛的遗传多样性，采用ISSR分子标记技术，分析了采自浙江平阳、瑞安、永嘉、乐清、温州等地温州水牛血液样本。从22条引物中筛选出9条多态性引物，9条ISSR引物共获得ISSR位点186个，其中多态性位点154个，多态位点百分率（PPL） 为82.80％，等位基因数（Na）为1.8420，有效等位基因数（Ne）为1.5702，Neis基因多样性（H）为0.3108，Shannons指数（I）为0.4687，均高于个体水平；种群内和种群间Neis基因多样性计算遗传分化水平（Gst）为0.1840，表明温州水牛种群间存在着较高的遗传多样性，遗传变异中有1840%发生于群体间。UPGMA聚类分析结果同样也表明温州水牛种群间的遗传分化水平较低，遗传变异主要存在于种群的个体间。

ISSR-PCR analysis on genetic diversity of Wenzhou buffalo

The genetic diversity and genetic structure of Wenzhou buffaloes distributed in Pingyang， Ruian， Yongjia， Yueqing and Wenzhou in Zhejiang province were analyzed by ISSR-PCR. A total of 154 polymorphic loci of 186 were amplified using 9 primers selected from 22 primers. Percentage of polymorphism loci （PPL） was 82.80％， observed number of alleles was 1.8420， effective number of alleles was 1.5702， Neis gene diversity was 0.3108， Shannons information index was 0.4687， all of the genetic parameters in population level were higher than individual level. Genetic differentiation level （Gst） calculated according to Neis gene diversity was 0.1840， which suggested that 18.40% genetic variation was present in populations. UPMGA cluster analysis in the strains of Wenzhou buffaloes also demonstrated that Wenzhou buffaloes has lower genetic diversity among the populations and the genetic variation mainly exists in the individual ones.