| 青海产菊粉酶菌株的筛选及分子鉴定 |
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| 引用本文： | 韩睿. 青海产菊粉酶菌株的筛选及分子鉴定[J]. 浙江农业学报, 2013, 25(4): 0 |
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| 作者姓名： | 韩睿 |
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| 作者单位： | 青海大学 农林科学院，青海省蔬菜遗传与生理重点实验室，青海 西宁 810016 |
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| 基金项目： | 青海大学中青年科研基金项目 |
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| 摘 要： | 从青海省种植菊芋的根际土壤中分离得到能产菊粉酶的菌株46株，从中筛选获得了1株产菊粉酶能力较高的菌株，酶活达到6.67 U·mL-1，且该菌株产菊粉酶类型为外切酶。通过对其rDNA-ITS的序列测定及分析，再结合形态学观察，将菌株鉴定为雅致放射毛霉Actinomucor elegans。
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| 关 键 词： | 菊粉酶 菌株筛选 菌种鉴定 青海 |
Screening and molecular identification of strain that produced inulinase in Qinghai |
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| HAN Rui. Screening and molecular identification of strain that produced inulinase in Qinghai[J]. Acta Agriculturae Zhejiangensis, 2013, 25(4): 0 |
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| Authors: | HAN Rui |
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| Affiliation: | Qinghai University Academy of Agriculture & Forestry，Qinghai Key Laboratory of Vegetable Genetics and Physiology，Xining 810016， China |
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| Abstract: | The 46 strains that produced inulase were screened from rhizosphere soil where Jerusalem artichoke was planted in Qinghai. One strain with high inulinase productivity was obtained through further screening and the enzyme activity was 6.67 U·mL-1. This inulinase was exo-inulinase. Through determination and analysis of the rDNA-ITS sequence， and analysis of morphology， the fungus was identified as Actinomucor elegans. |
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| Keywords: | inulinase strain screening strain identification Qinghai |
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