| 油菜PEPase基因的克隆及其对应RNAi载体的构建 |
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| 引用本文： | 张银波,江木兰,胡小加.油菜PEPase基因的克隆及其对应RNAi载体的构建[J].中国油料作物学报,2005,27(1):1-4. |
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| 作者姓名： | 张银波 江木兰 胡小加 |
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| 作者单位： | 中国农业科学院油料作物研究所,农业部油料作物遗传改良重点实验室,湖北,武汉,430062 |
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| 摘 要： | 磷酸烯醇式丙酮酸羧化酶(PEPase)是控制油菜蛋白质/油脂含量比例的一个关键酶.采用RT-PCR方法从甘蓝型油菜中双4号中扩增出PEPase基因cDNA片段,与已发表的PEPase基因(登录号为D13987)序列同源性为98%.根据RNA 干扰(RNAi)目标序列的选取原则选取两段长度约为400bp的DNA序列,分别克隆到RNAi载体pHBM1301中,构建了油菜中对应于PEPase基因的RNAi载体pHBM1301-PEP1和pHBM1301-PEP2.
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| 关 键 词： | 磷酸烯醇式丙酮酸羧化酶 |
| 文章编号： | 1007-9084(2005)01-0001-04 |
| 修稿时间： | 2004年9月6日 |
Molecular cloning of the PEPase gene from Brassica napus and the construction of its corresponding RNAi vectors |
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| ZHANG Yin-bo,JIANG Mu-lan,HU Xiao-jia.Molecular cloning of the PEPase gene from Brassica napus and the construction of its corresponding RNAi vectors[J].Chinese Journal of Oil Crop Sciences,2005,27(1):1-4. |
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| Authors: | ZHANG Yin-bo JIANG Mu-lan HU Xiao-jia |
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| Abstract: | Phosphoenolpyruvate carboxylase(PEPase) plays an important role in the control of the ratio of the protein/lipid content in rape seed (Brassica napus). The cDNA fragment of PEPase gene was cloned from B.napus zhongshuang No.4 by RT-PCR. Sequence analysis of the fragment suggested that it possessed 98% homology with the PEPase gene(D14985). In order to down-regulate the expression of PEPase gene, two 400bp fragments were cloned into the Sap I site of RNAi vector pHBM1301 and two recombinant plasmids pHBM1301-PEP1 and pHBM1301-PEP2 were obtained. |
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| Keywords: | RT-PCR RNAi |
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