| 洋葱花青素合成酶基因的克隆和序列分析 |
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| 引用本文： | 缪军,刘冰江,杨妍妍,霍雨猛,张一卉,霍凤梅,修景润,吴雄. 洋葱花青素合成酶基因的克隆和序列分析[J]. 山东农业科学, 2010, 0(1): 1-5 |
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| 作者姓名： | 缪军 刘冰江 杨妍妍 霍雨猛 张一卉 霍凤梅 修景润 吴雄 |
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| 作者单位： | 山东省农业科学院蔬菜研究所,山东,济南,250100 |
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| 基金项目： | 国家自然科学基金项目(30871706);;山东省农业科学院创新基金项目(2006YCX015) |
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| 摘 要： | 采用PCR法克隆了洋葱的花青素合成酶基因AcANS，并对其进行序列比对和生物信息学分析。结果表明，其开放阅读框为1059bp，编码352个氨基酸残基；具有一个富含AT的内含子，符合GT—AG规则；其蛋白质第210～309肽段含有20G—Fe（Ⅱ）加氧酶家族基因的结构域。
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| 关 键 词： | 洋葱 花青素合成酶基因 基因克隆 序列分析 |
Cloning and Sequence Analysis of Anthocyanidin Synthase Gene in Onion |
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| MIAO Jun,LIU Bing-jiang,YANG Yan-yan,HUO Yu-meng,ZHANG Yi-hui,HUO Feng-mei,XIU Jing-run,WU Xiong. Cloning and Sequence Analysis of Anthocyanidin Synthase Gene in Onion[J]. Shandong Agricultural Sciences, 2010, 0(1): 1-5 |
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| Authors: | MIAO Jun LIU Bing-jiang YANG Yan-yan HUO Yu-meng ZHANG Yi-hui HUO Feng-mei XIU Jing-run WU Xiong |
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| Affiliation: | Institute of Vegetables;Shandong Academy of Agricultural Sciences;Jinan 250100;China |
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| Abstract: | Bulb color is an important economic trait in onion(Allium cepa).The anthocyanidin synthase gene AcANS in onion was cloned by PCR,and its sequence alignment and bioinformatics analysis were carried out.The open reading frame(ORF) of AcANS gene was 1 059 bp,and it encoded a protein with 352 amino acids.An AT-rich intron of 107 bp,obeying the GT-AG rule,was in the DNA sequence.The 2OG-Fe(II) domain of oxygenase family gene was in the peptide from 210 to 309. |
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| Keywords: | Onion Anthocyanidin synthase gene Gene clone Sequence analysis |
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