Somatic embryogenesis and histological analysis from zygotic embryos in Vitis vinifera L. 'Moldova' |
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基金项目: | 引进国际先进农业科技计划(948计划) |
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摘 要: |
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关 键 词: | 林业 失水量 细胞分裂生长 组织学 植物 |
Somatic embryogenesis and histological analysis from zygotic embryos in Vitis vinifera L. 'Moldova' |
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Authors: | LI Hui LI Feng-lan DU Jing-chuan LU Hai HE Zheng-quan |
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Institution: | 1. College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, P. R. China 2. Biotechnology Research Center, Three Gorges University, Yichang 443000, P. R. China |
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Abstract: | We examined the somatic embryogenesis from and histological studies of zygotic embryos of seeds in European Grape 'Moldova' (Vitis vinifera L. 'Moldova'). Primary calli were initiated on Nitsch and Nitsch (NN) medium supplemented with 1.0mg'L-1 2,4-D and 0.5 mg'L-1 6-BA. Embryogenic calli were produced upon transfer to a NN medium with 0.5 mg-L-1 6-BA and 2 mg.L-1 NAA and somatic embryos were obtained on a half strength MS medium without plant growth regulators. During the somatic embryo germination, an addition of 1.0 mg.L-1 6-BA in the medium could accelerate somatic embryos to develop into normal plants and increase the conversion rate from 0 to 43.3%. Histological studies of embryogenic calli and somatic embryos demonstrated dy-namic changes of proteins and starch grains. The developmental processes of somatic embryos were similar to those of zygotic em-bryos, including typical epiderma, cotyledon primordium and vascular tissue. |
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Keywords: | zygotic embryo somatic embryo histology Vitis vinifera L |
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