大孔吸附树脂纯化金樱根总黄酮的研究 |
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引用本文: | 吴功庆,易运红,邹梅清,田素英,赵增阳.大孔吸附树脂纯化金樱根总黄酮的研究[J].安徽农业科学,2011,39(20):12143-12144,12146. |
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作者姓名: | 吴功庆 易运红 邹梅清 田素英 赵增阳 |
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作者单位: | 1. 广东药学院中山校区,广东,中山,528458 2. 中山大学生命科学学院,广东,广州,510275 |
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基金项目: | 2009年中山市华南现代中药城专项发展资金 |
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摘 要: | 目的]筛选出一种树脂,并优化大孔吸附树脂纯化金樱根总黄酮成分的工艺。方法]比较ADS-7、HPD300、AB-8、D-101型4种大孔吸附树脂对金樱根粗总黄酮提取液的吸附-解吸效果,考察上样液浓度、洗脱液浓度对解吸效果的影响。结果]AB-8型大孔树脂对金樱根总黄酮成分吸附分离效果最佳;其纯化金樱根最佳条件为以20.0 ml浓度为0.200 mg/ml的样液进行吸附,用4 BV 50%乙醇进行洗脱。最佳工艺下,金樱根总黄酮的精制度为205.40%。结论]通过该研究得到的方法可获得高纯度的金樱根总黄酮。
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关 键 词: | 金樱根 大孔吸附树脂 总黄酮 分离纯化 |
Study on Purification of Total Flavonoids from Root of Rosa laevigata Michx.by Macroporous Resin |
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Institution: | WU Gong-qing et al(Zhongshan Campus,Guangdong Pharmaceutical University,Zhongshan,Guangdong 528458) |
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Abstract: | Objective] To obtain a suitable macroporous resin,and to optimize its purification process for total flavonoids of root of Rosa laevigata Michx..Method] The adsorption and desorption effect of four resins like ADS-7,HPD300,AB-8 and D-101 were comparatively studied and effect of concentration of loading solution and eluent concentration were investigated.Result] AB-8 resin was the optimum purification resin for total flavonoids of root of Rosa laevigata Michx.,and the optimum purification conditions were:loading concentration of 0.200 mg/ml,loading volume of 20.0 ml,eluent of 50% ethanol of 4 BV.Under the optimal conditions,the purification rate of total flavonoids of root of Rosa laevigata Michx.reached as high as 205.40%.Conclusion] The high quality total flavonoids from root of Rosa laevigata Michx.was obtained in this work. |
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Keywords: | Root of Rosa laevigata Michx Macroporous resin Total flavonoids Isolation and purification |
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