葛根总黄酮提取物成分的HPLC测定

目的]测定葛根总黄酮提取物有效成分葛根素、大豆苷含量。方法]HPLC色谱条件为：色谱柱为C18柱;流动相为乙腈-水（25∶75,V/V）;检测波长为250 nm;流速为0.8 ml/min;柱温为室温;进样量为4μl。结果]在测定范围内,葛根素、大豆苷对照品线性关系良好;葛根中葛根素、大豆苷平均含量分别为5.41、7.50μg/ml。结论]该研究为更好的利用葛根提供了依据。

Determination of Constituents of Total Flavonoids Extracts of RADIX PUERARIAE by HPLC

Objective] To establish a method for determining puerarin and daidzin from total flavonoids extracts of RADIX PUERARIAE.Method] HPLC conditions were:chromatographic column of C18 column,mobile phase of acetonitrile-water(25∶75,V/V),detection wavelength of 250 nm,flow rate of 0.8 ml/min,column temperature of room temperature and sampling amount of 4 μl.Result] In determining range,puerarin and daidzin showed good relationship to concentration,respectively.Total flavonoids extracts from RADIX PUERARIAE were 5.41 and 7.50 μg/ml in puerarin and daidzin individually.Conclusion] This case provided basis for better utilization of RADIX PUERARIAE.