反相高效液相梯度洗脱法测定药材中雷公藤甲素的含量

目的]通过高效液相色谱法建立测定雷公藤甲素含量的方法,控制药材质量,为保证临床用药的有效性和安全性提供依据。方法]采用反相高效液相层析（RP-HPLC）梯度洗脱法建立雷公藤药材中雷公藤甲素的定量方法。采用AgilentC18柱（2.1mm×150mm,3μm）;乙腈-水二元梯度洗脱,0～8min（10：90～25：75,V：V）,8～33min（25：75～35：65,V：V）,33～40min（35：65～95：5,V：V）,40～48min（95：5,V：V）,48～55min（10：90,V：V）;流速：1.0ml/min;检测波长：220nm。结果]采用RP-HPLC梯度洗脱法不仅快速有效地实现了药材中雷公藤甲素的良好分离,而且能够有效防止分析过程中的拖尾现象,较准确地测定雷公藤甲素的含量。雷公藤甲素的线性范围为25～2000μg/ml（r=0.9999）,该方法回收率为97.48%（RSD=1.55%）。结论]该方法灵敏度高,准确可靠,重现性好,结果稳定。

Determination of Triptolide Content in Tripterygium wilfordii by RP-HPLC Gradient Elution Method

Objective]Based on RP-HPLC gradient elution method,the determination method of triptolide content was established and the quality of medicinal materials was controlled to provide basis for ensuring the effectiveness and safety of clinical medication.Methods] RP-HPLC gradient elution method was used to establish quantitative approach of triptolide in Tripterygium wilfordii,AgilentC18 column was used with a mobile phase of acetonitrile-water,0-8 min(10:90-25:75,V:V),8-33 min(25:75-35:65,V:V),33-40 min(35:65-95:5,V:V),40-48 min(95:5,V:V),48-55 min(10:90,V:V) gradient elution,flow velocity was 1.0 ml/min and the wavelength of detector was set at 220 nm.Results] Based on RP-HPLC gradient elution method,can not only realize the separation of triptolide in Tripterygium wilfordii effectively,but also can control the delay phenomenon effectively during the analysis process,thus the triptolide content can be determined exactly.The linear ranges of triptolide was 25-2000 μg/ml(r = 0.9999),the average recovery rates were 97.48%(RSD = 1.55%).Conclusion]The method was rapid,accurate,sensitive and reliable.