响应面法优化茅苍术多糖的提取工艺 |
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引用本文: | 徐秀泉,于荣敏,刘柯,罗明. 响应面法优化茅苍术多糖的提取工艺[J]. 安徽农业科学, 2011, 39(20): 12082-12085 |
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作者姓名: | 徐秀泉 于荣敏 刘柯 罗明 |
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作者单位: | 1. 江苏大学药学院,江苏,镇江,212013 2. 暨南大学药学院,广东,广州,510632 |
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基金项目: | 江苏大学高级人才专项补助,江苏大学大学生科研立项项目 |
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摘 要: | [目的]利用响应面分析法优化茅苍术多糖的提取工艺。[方法]根据中心组合设计原理,以提取温度、提取时间、料液比三个因素为自变量,多糖的提取得率为响应值,设计了3因素3水平响应面分析试验,来确定茅苍术多糖提取的最佳工艺条件。[结果]茅苍术多糖提取的最佳工艺条件为:提取温度87℃,料液比1∶22,提取时间3.1 h。在此最佳条件下,茅苍术多糖的提取率为2.12%。[结论]该研究对大规模提取茅苍术多糖有一定理论指导价值。
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关 键 词: | 茅苍术(RHIZOMA AREACTYLODIS LANCEAE) 多糖 提取 响应面 |
Optimization of Extraction Process of Polysaccharides from,RHIZOMA AREACTYLODIS LANCEAE by Response Surface Methodology |
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Affiliation: | XU Xiu-quan et al (College of Pharmacy,Jiangsu University,Zhenjiang,Jiangsu 212013) |
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Abstract: | [Objective] To optimize the extraction process of polysaccharides from RHIZOMA AREACTYLODIS LANCEAE by response surface methodology.[Method] Using extraction temperature,solid-liquid ratio and extraction time as independent variables and extraction rate of polysaccharides as response value,response surface methodology with three-factors and three-levels was carried out for optimizing the extraction process of polysaccharides from RHIZOMA AREACTYLODIS LANCEAE on the basis of central composite design principles.[Result] The optimum extraction conditions of polysaccharides were as follows: extraction temperature of 87 ℃,solid-liquid ratio of 1∶22 and extraction time of 3.1 h.Under this condition,the extraction rate of polysaccharides was 2.12%.[Conclusion] The study could provide a certain theoretical direction for extracting polysaccharides from RHIZOMA AREACTYLODIS LANCEAE on a large scale. |
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Keywords: | RHIZOMA AREACTYLODIS LANCEAE Polysaccharides Extraction Response surface methodology |
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