3种抑菌剂对蛇足石杉内生真菌黑曲霉的抑制效果研究

目的]探索3种抑菌剂对蛇足石杉内生真菌黑曲霉的抑制效果。方法]采用平板涂布法分离获得供试菌,用菌丝生长速率法测定3种抑菌剂对黑曲霉的抑制效果。结果]两性霉素B、苯甲酸钠、肉桂醛对黑曲霉的最低抑菌浓度分别为512.000 00 mg/L、4.00g/L、0.500 00 mg/L;EC50分别为1.910 00、150.000 00、0.120 00 mg/L,EC90分别为88.220 00、730.000 00、0.330 00 mg/L;3种抑菌剂中较优的抑菌剂为两性霉素B。结论]该研究获得了3种抑菌剂的最低抑菌浓度,为蛇足石杉组织培养的深入研究提供了试验基础。

Study on the Inhibitive Effect of Three Antimicrobial Agents on Endophytic Fungi Aspergillus niger of Huperzia serrata

Objective] To explore the inhibitory effect of three antimicrobial agents against Aspergillus niger,a dominant endophytic fungus in Huperzia serrata.Method] A.niger was isolated by plate coating method and the inhibitive effect of three antimicrobial agents on A.niger was obtained by mycelial growth rate method.The effect of three antimicrobial agents on H.serrata was conducted by inoculating stem segments of H.serrata on MS media with antimicrobial agents or by inoculating stem segments which were soaked in the minimum concentrations of antimicrobial agents for one or two days on MS media without antimicrobial agents.Result] The minimal inhibitory concentrations of amphotericin B,sodium benzoate and cinnamic aldehyde for A.niger were 512.00 000 mg / L,4.00 g/L and 0.500 000 mg/L,respectively.Their EC50 were 1.910 00,150.00 000 and 0.120 00 mg/L,respectively and Their EC90 were 88.22 mg/L,730.00 mg/L and 0.330 00 mg/L respectively.Amphotericin B was the optimum antimicrobial agent for A.niger in Huperzia serrata.Conclusion] The minimum inhibitory concentrations of three antimicrobial agents for A.niger were obtained,which providedexperimental basis for tissue culture of H.serrata.