| 罗布麻种子诱导分化培养基的筛选 |
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| 引用本文： | 刘萍. 罗布麻种子诱导分化培养基的筛选[J]. 安徽农业科学, 2011, 39(21): 12853+12856-12853,12856 |
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| 作者姓名： | 刘萍 |
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| 作者单位： | 吉林农业科技学院高职学院,吉林左家,132109 |
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| 摘 要： | [目的]筛选罗布麻种子诱导分化的最佳培养基。[方法]以MS培养基为基础培养基,选择无污染的带皮罗布麻种芽（露白）作为外植体,考察不同浓度NAA对罗布麻种子诱导分化的影响,筛选出最佳的培养基。[结果]不同浓度的NAA均能促进罗布麻种子成活,且不同浓度见无显著性差异。罗布麻种子的最佳培养基为：MS＋0.02mg/LNAA＋1.00mg/LIBA＋10g/L琼脂＋30g/L蔗糖。[结论]该研究为罗布麻的规模化生产奠定了基础。
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| 关 键 词： | 罗布麻 种子 诱导分化 培养基 |
Screen of Differentiation Medium of Seed from Apocynum venetum L. |
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| LIU Ping. Screen of Differentiation Medium of Seed from Apocynum venetum L.[J]. Journal of Anhui Agricultural Sciences, 2011, 39(21): 12853+12856-12853,12856 |
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| Authors: | LIU Ping |
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| Affiliation: | LIU Ping(Jilin Academy of Agricultural Science and Technology Vocational College,Zuojia,Jilin 132109) |
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| Abstract: | [Objective]To screen out a optimal medium for differentiation of seed from Apocynum venetum L..[Method]Using MS as the basic medium and free bulbils of Apocynum venetum as explants,effect of NAA concentration on differentiation of seed from Apocynum venetum was investigated,obtaining the optimum medium.[Result]NAA favored the survival of Apocynum venetum seed,and was not significant in different concentration.The optimal medium for differentiation of Apocynum venetum seed was:MS + 0.02 mg/L NAA + 1.00 mg/L IBA + 10 g/L agar + 30 g/L cane sugar.[Conclusion]This case provided basis for the industrialization production of Apocynum venetum. |
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| Keywords: | Apocynum venetum L. Seed Differentiation Medium |
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