魔芋胶酶解工艺条件的优化 |
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引用本文: | 徐丽萍,吴正勇,任富修,张永奎. 魔芋胶酶解工艺条件的优化[J]. 安徽农业科学, 2011, 39(21): 13058-13059 |
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作者姓名: | 徐丽萍 吴正勇 任富修 张永奎 |
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作者单位: | 1. 四川化工职业技术学院,四川泸州646005;四川大学化学工程学院,四川成都610065 2. 四川化工职业技术学院,四川泸州,646005 3. 成都永安制药公司,四川成都,610041 4. 四川大学化学工程学院,四川成都,610065 |
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摘 要: | [目的]探讨在中试条件下利用中性β-甘露聚糖酶水解魔芋胶制备低聚甘露糖的工艺条件。[方法]在单因素试验条件下,研究了利用枯草芽孢杆菌所产生的中性β-甘露聚糖酶对魔芋胶进行水解来制备低聚甘露糖的工艺条件。[结果]酶法水解魔芋胶生产低聚甘露糖时,优化的酶解条件为:pH6.0,魔芋胶浓度125g/L,酶量200IU/g魔芋胶,酶解温度50℃,酶解时间3h。[结论]该研究可极大地提高魔芋胶的附加值。
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关 键 词: | β-甘露聚糖酶 低聚甘露糖 魔芋胶 |
Study on the Optimization of the Enzymatic Hydrolysis Conditions of Konjak Gum |
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Affiliation: | XU Li-ping et al(Sichuan College of Chemical Technology,Luzhou,Sichuan 646005) |
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Abstract: | [Objective]The research aimed to discuss the technology conditions of producing mannose-oligosaccharides by hydrolyzing konjak gum using neutral β-mannanase under middle-test conditions.[Method] Under the conditions of single factor test,hydrolytic conditions of konjak gum for producing mannose-oligosaccharides were studied with neutral β-mannanase from Bacillus subtilis.[Result]When konjak gum was hydrolyzed by enzyme method for producing mannose-oligosaccharides,the optimized enzymatic hydrolysis conditions were as follows:pH of 6.0,konjak gum concentration of 150 g/L,β-mannanase dosage of 200 IU/g konjak gum,enzymatic hydrolytic temperature of 50 ℃ and hydrolytic time of 3 h.[Conclusion]The research could greatly enhance the additive value of konjak gum. |
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Keywords: | β-mannanase mannose-oligosaccharides konjak gum |
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